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Bioluminescent sensor proteins for point-of-care therapeutic drug monitoring.
Journal article

Bioluminescent sensor proteins for point-of-care therapeutic drug monitoring.

  • Griss R 1] École Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering, Lausanne, Switzerland. [2] École Polytechnique Fédérale de Lausanne, Institute of Bioengineering, Lausanne, Switzerland. [3] National Centre of Competence in Research in Chemical Biology, Lausanne, Switzerland.
  • Schena A 1] École Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering, Lausanne, Switzerland. [2] École Polytechnique Fédérale de Lausanne, Institute of Bioengineering, Lausanne, Switzerland. [3] National Centre of Competence in Research in Chemical Biology, Lausanne, Switzerland.
  • Reymond L 1] École Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering, Lausanne, Switzerland. [2] École Polytechnique Fédérale de Lausanne, Institute of Bioengineering, Lausanne, Switzerland. [3] National Centre of Competence in Research in Chemical Biology, Lausanne, Switzerland.
  • Patiny L cole Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering, Lausanne, Switzerland.
  • Werner D Clinical Chemistry Laboratory, Service of Biomedicine, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland.
  • Tinberg CE University of Washington, Department of Biochemistry, Seattle, Washington, USA.
  • Baker D University of Washington, Department of Biochemistry, Seattle, Washington, USA.
  • Johnsson K 1] École Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering, Lausanne, Switzerland. [2] École Polytechnique Fédérale de Lausanne, Institute of Bioengineering, Lausanne, Switzerland. [3] National Centre of Competence in Research in Chemical Biology, Lausanne, Switzerland.
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  • 2014-06-09
Published in:
  • Nature chemical biology. - 2014
Anti-Arrhythmia Agents
Anticonvulsants
Antineoplastic Agents
Biosensing Techniques
Drug Monitoring
Escherichia coli
Gene Expression
Humans
Image Processing, Computer-Assisted
Immunosuppressive Agents
Luminescent Proteins
Photography
Point-of-Care Systems
Protein Engineering
Recombinant Fusion Proteins
English For many drugs, finding the balance between efficacy and toxicity requires monitoring their concentrations in the patient's blood. Quantifying drug levels at the bedside or at home would have advantages in terms of therapeutic outcome and convenience, but current techniques require the setting of a diagnostic laboratory. We have developed semisynthetic bioluminescent sensors that permit precise measurements of drug concentrations in patient samples by spotting minimal volumes on paper and recording the signal using a simple point-and-shoot camera. Our sensors have a modular design consisting of a protein-based and a synthetic part and can be engineered to selectively recognize a wide range of drugs, including immunosuppressants, antiepileptics, anticancer agents and antiarrhythmics. This low-cost point-of-care method could make therapies safer, increase the convenience of doctors and patients and make therapeutic drug monitoring available in regions with poor infrastructure.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/148096
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