A roadmap for interpreting (13)C metabolite labeling patterns from cells.
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Buescher JM
Vesalius Research Center, VIB, Leuven, Belgium; Department of Oncology, KU Leuven, Leuven, Belgium.
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Antoniewicz MR
Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE, USA.
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Boros LG
Department of Pediatrics, UCLA School of Medicine, Los Angeles Biomedical Research Institute at the Harbor-UCLA Medical Center and Sidmap, LLC, Los Angeles, CA, USA.
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Burgess SC
Advanced Imaging Research Center-Division of Metabolic Mechanisms of Disease and Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX, USA.
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Brunengraber H
Department of Nutrition, Case Western Reserve University School of Medicine, Cleveland, OH, USA.
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Clish CB
Broad Institute of Harvard and MIT, Cambridge, MA, USA.
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DeBerardinis RJ
Children's Medical Center Research Institute, UT Southwestern Medical Center, Dallas, TX, USA.
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Feron O
Pole of Pharmacology and Therapeutics (FATH), Institut de Recherche Expérimentale et Clinique (IREC), Université catholique de Louvain, Brussels, Belgium.
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Frezza C
MRC Cancer Unit, University of Cambridge, Hutchison/MRC Research Centre, Cambridge Biomedical Campus, Cambridge, UK.
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Ghesquiere B
Vesalius Research Center, VIB, Leuven, Belgium; Department of Oncology, KU Leuven, Leuven, Belgium.
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Gottlieb E
Cancer Research UK, Beatson Institute, Glasgow, UK.
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Hiller K
Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Esch-Belval, Luxembourg.
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Jones RG
Goodman Cancer Research Centre, Department of Physiology, McGill University, Montreal, QC, Canada.
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Kamphorst JJ
Institute of Cancer Sciences, University of Glasgow, Glasgow, UK.
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Kibbey RG
Internal Medicine, Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT, USA.
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Kimmelman AC
Division of Genomic Stability and DNA Repair, Department of Radiation Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.
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Locasale JW
Division of Nutritional Sciences, Cornell University, Ithaca, NY, USA.
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Lunt SY
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, USA.
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Maddocks OD
Cancer Research UK, Beatson Institute, Glasgow, UK.
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Malloy C
Advanced Imaging Research Center-Division of Metabolic Mechanisms of Disease and Department of Radiology, The University of Texas Southwestern Medical Center, Dallas, TX, USA.
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Metallo CM
Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
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Meuillet EJ
L'Institut des Technologies Avancées en Sciences du Vivant (ITAV), Toulouse Cedex 1, France; The University of Arizona Cancer Center, and Department of Nutritional Sciences, The University of Arizona, Tucson, AZ, USA.
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Munger J
Department of Biochemistry, University of Rochester Medical Center, Rochester, NY, USA; Department of Biophysics, University of Rochester Medical Center, Rochester, NY, USA.
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Nöh K
Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, Germany.
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Rabinowitz JD
Department of Chemistry and Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA.
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Ralser M
Cambridge Systems Biology Centre and Department of Biochemistry, University of Cambridge, Cambridge, UK; Division of Physiology and Metabolism, MRC National Institute for Medical Research, London, UK.
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Sauer U
Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
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Stephanopoulos G
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
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St-Pierre J
Goodman Cancer Research Centre, and Department of Biochemistry, McGill University, Montreal, Quebec, Canada.
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Tennant DA
School of Cancer Sciences, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham, UK.
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Wittmann C
Institute of Systems Biotechnology, Saarland University, Saarbrücken, Germany.
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Vander Heiden MG
Koch Institute for Integrative Cancer Research at Massachusetts Institute of Technology, Broad Institute of Harvard and MIT, Cambridge, MA, USA; Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.
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Vazquez A
Cancer Research UK, Beatson Institute, Glasgow, UK.
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Vousden K
Cancer Research UK, Beatson Institute, Glasgow, UK.
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Young JD
Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, USA; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA.
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Zamboni N
Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
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Fendt SM
Vesalius Research Center, VIB, Leuven, Belgium; Department of Oncology, KU Leuven, Leuven, Belgium. Electronic address: sarah-maria.fendt@vib-kuleuven.be.
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Published in:
- Current opinion in biotechnology. - 2015
English
Measuring intracellular metabolism has increasingly led to important insights in biomedical research. (13)C tracer analysis, although less information-rich than quantitative (13)C flux analysis that requires computational data integration, has been established as a time-efficient method to unravel relative pathway activities, qualitative changes in pathway contributions, and nutrient contributions. Here, we review selected key issues in interpreting (13)C metabolite labeling patterns, with the goal of drawing accurate conclusions from steady state and dynamic stable isotopic tracer experiments.
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Language
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Open access status
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green
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Persistent URL
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https://folia.unifr.ch/global/documents/143605
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