Journal article
Cryo-electron microscopy of vitreous sections of native biological cells and tissues.
- Al-Amoudi A Laboratoire d'Analyse Ultrastructurale, Bâtiment de Biologie, Université de Lausanne, CH-1015, Switzerland.
- Norlen LP
- Dubochet J
- 2004-09-15
Published in:
- Journal of structural biology. - 2004
Animals
Animals, Newborn
Biopsy
Cryoelectron Microscopy
Cyanobacteria
Epidermis
Freezing
Humans
Male
Mice
Microtomy
Skin
Specimen Handling
English
Cryo-electron microscopy of vitreous sections (CEMOVIS) is, in principle, the ultimate method of specimen preparation. It consists in ultra-rapid cooling of a sizable sample of biological material that is cut into thin sections. These are subsequently observed at low temperature in their fully hydrated vitreous state. Here, we show that CEMOVIS reveals the native state of cells and tissues with unprecedented quality and resolution. What is seen differs considerably from what conventional electron microscopy has shown previously and it is seen with more details. Our findings are demonstrated with images of cyanobacteria and skin.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1016/j.jsb.2004.03.010
- PMID 15363793
- Persistent URL
- https://folia.unifr.ch/global/documents/136256
Statistics
Document views: 7
File downloads: