Journal article
Design and synthesis of a novel DNA-encoded chemical library using Diels-Alder cycloadditions.
- Buller F Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, Wolfgang-Pauli-Strasse 10, CH-8093 Zurich, Switzerland.
- Mannocci L
- Zhang Y
- Dumelin CE
- Scheuermann J
- Neri D
- 2008-08-05
Published in:
- Bioorganic & medicinal chemistry letters. - 2008
Base Sequence
Combinatorial Chemistry Techniques
DNA
Gene Library
Molecular Sequence Data
Molecular Structure
Oligonucleotides
Streptavidin
English
DNA-encoded chemical libraries are increasingly being employed for the identification of binding molecules to protein targets of pharmaceutical relevance. Here, we describe the synthesis and characterization of a DNA-encoded chemical library, consisting of 4000 compounds generated by Diels-Alder cycloaddition reactions. The compounds were encoded with unique DNA fragments which were generated through a stepwise assembly process and serve as amplifiable bar codes for the identification and relative quantification of library members.
- Language
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- English
- Open access status
- closed
- Identifiers
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- DOI 10.1016/j.bmcl.2008.07.038
- PMID 18674904
- Persistent URL
- https://folia.unifr.ch/global/documents/132659
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