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Large-scale transient expression in mammalian cells for recombinant protein production.
Journal article

Large-scale transient expression in mammalian cells for recombinant protein production.

  • Wurm F Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland.
  • Bernard A
  • 1999-04-21
Published in:
  • Current opinion in biotechnology. - 1999
Adenoviridae
Alphavirus
Animals
Cell Culture Techniques
Cell Line
Chlorocebus aethiops
Cloning, Molecular
Cricetinae
Genetic Vectors
Humans
Recombinant Proteins
Vaccinia virus
English Large-scale transient expression from mammalian cells is a new technology. Breakthroughs have been achieved for non-viral delivery methods: transfections can now be done at the 1-10 L scale with mammalian cells grown in suspension. Production of 1-20 mg/L of recombinant protein have been obtained in stirred bioreactors. Modified alphaviruses have provided a fast and efficient expression technology based on viral vectors.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/126399
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