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Use of flow cytometric methods for single-cell analysis in environmental microbiology.
Journal article

Use of flow cytometric methods for single-cell analysis in environmental microbiology.

  • Czechowska K Department of Fundamental Microbiology, Bâtiment Biophore, University of Lausanne, 1015 Lausanne, Switzerland. kamilarenata.czechowska@unil.ch
  • Johnson DR
  • van der Meer JR
  • 2008-06-20
Published in:
  • Current opinion in microbiology. - 2008
Bacteria
Environmental Microbiology
Flow Cytometry
In Situ Hybridization, Fluorescence
English Flow cytometry (FCM) is emerging as an important tool in environmental microbiology. Although flow cytometry applications have to date largely been restricted to certain specialized fields of microbiology, such as the bacterial cell cycle and marine phytoplankton communities, technical advances in instrumentation and methodology are leading to its increased popularity and extending its range of applications. Here we will focus on a number of recent flow cytometry developments important for addressing questions in environmental microbiology. These include (i) the study of microbial physiology under environmentally relevant conditions, (ii) new methods to identify active microbial populations and to isolate previously uncultured microorganisms, and (iii) the development of high-throughput autofluorescence bioreporter assays.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/122806
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