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Conformational manipulation of scale-up prepared single-chain polymeric nanogels for multiscale regulation of cells.

  • Chen X Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong, 999077, Hong Kong.
  • Li R Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong, 999077, Hong Kong.
  • Wong SHD Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong, 999077, Hong Kong.
  • Wei K Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomimetic Membranes and Textiles, Lerchenfeldstrasse 5, CH-9014, St. Gallen, Switzerland.
  • Cui M Beijing Genomic Institute-Shenzhen, Shenzhen, 518083, China.
  • Chen H The State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai, 200433, China.
  • Jiang Y Institute of Textiles & Clothing, The Hong Kong Polytechnic University, Hong Kong, 999077, Hong Kong.
  • Yang B Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong, 999077, Hong Kong.
  • Zhao P Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong, 999077, Hong Kong.
  • Xu J Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, 310016, China.
  • Chen H Shenzhen Key Laboratory of Special Functional Materials, College of Materials Science and Engineering, Shenzhen University, Shenzhen, 518060, China.
  • Yin C Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong, 999077, Hong Kong.
  • Lin S Department of Orthopaedics & Traumatology, Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, 999077, Hong Kong.
  • Lee WY Department of Orthopaedics & Traumatology, Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, 999077, Hong Kong.
  • Jing Y Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong, 999077, Hong Kong.
  • Li Z The State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai, 200433, China.
  • Yang Z Department of Orthopaedics & Traumatology, Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, 999077, Hong Kong.
  • Xia J Department of Chemistry, The Chinese University of Hong Kong, Hong Kong, 999077, Hong Kong.
  • Chen G The State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai, 200433, China.
  • Li G Department of Orthopaedics & Traumatology, Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, 999077, Hong Kong.
  • Bian L Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong, 999077, Hong Kong. lbian@cuhk.edu.hk.
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  • 2019-06-22
Published in:
  • Nature communications. - 2019
Biocompatible Materials
Cell Differentiation
Cell Engineering
Cell Line
Drug Carriers
Humans
Hydrogels
Mesenchymal Stem Cells
Molecular Conformation
Nanoparticles
Polymers
RNA, Small Interfering
English Folded single chain polymeric nano-objects are the molecular level soft material with ultra-small size. Here, we report an easy and scalable method for preparing single-chain nanogels (SCNGs) with improved efficiency. We further investigate the impact of the dynamic molecular conformational change of SCNGs on cellular interactions from molecular to bulk scale. First, the supramolecular unfoldable SCNGs efficiently deliver siRNAs into stem cells as a molecular drug carrier in a conformation-dependent manner. Furthermore, the conformation changes of SCNGs enable dynamic and precise manipulation of ligand tether structure on 2D biomaterial interfaces to regulate the ligand-receptor ligation and mechanosensing of cells. Lastly, the dynamic SCNGs as the building blocks provide effective energy dissipation to bulk biomaterials such as hydrogels, thereby protecting the encapsulated stem cells from deleterious mechanical shocks in 3D matrix. Such a bottom-up molecular tailoring strategy will inspire further applications of single-chain nano-objects in the biomedical area.
Language
  • English
Open access status
gold
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Persistent URL
https://folia.unifr.ch/global/documents/112394
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