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Alignment of collagen fiber in knitted silk scaffold for functional massive rotator cuff repair.

  • Zheng Z Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Zhejiang 310000, China; Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang 310009, China; Department of Orthopedic Surgery, The Children's Hospital, School of Medicine, Zhejiang University, Zhejiang 310052, China.
  • Ran J Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang 310009, China; Department of Orthopedics Research Institute of Zhejiang University, Zhejiang, China.
  • Chen W Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang 310009, China; Department of Orthopedics Research Institute of Zhejiang University, Zhejiang, China.
  • Hu Y Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Zhejiang 310000, China; Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang 310009, China.
  • Zhu T Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang 310009, China.
  • Chen X Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Zhejiang 310000, China; Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, School of Medicine, Zhejiang University, Zhejiang 310009, China; China Orthopaedic Regenerative Medicine (CORMed), Hangzhou, China.
  • Yin Z Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Zhejiang 310000, China; Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, School of Medicine, Zhejiang University, Zhejiang 310009, China.
  • Heng BC Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong.
  • Feng G Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang 310009, China.
  • Le H Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang 310009, China; Department of Orthopedics Research Institute of Zhejiang University, Zhejiang, China.
  • Tang C Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang 310009, China; Department of Orthopedics Research Institute of Zhejiang University, Zhejiang, China.
  • Huang J Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang 310009, China; Department of Orthopedics Research Institute of Zhejiang University, Zhejiang, China.
  • Chen Y Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang 310009, China; Department of Orthopedics Research Institute of Zhejiang University, Zhejiang, China.
  • Zhou Y Department of Sports Medicine, School of Medicine, Zhejiang University, Zhejiang 310000, China; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang 310003, China.
  • Dominique P Laboratory of Biomechanical Orthopedics, EPFL, Lausanne, Switzerland.
  • Shen W Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Zhejiang 310000, China; Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang 310009, China; Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, School of Medicine, Zhejiang University, Zhejiang 310009, China; Department of Sports Medicine, School of Medicine, Zhejiang University, Zhejiang 310000, China; Laboratory of Biomechanical Orthopedics, EPFL, Lausanne, Switzerland; China Orthopaedic Regenerative Medicine (CORMed), Hangzhou, China; Department of Orthopedics Research Institute of Zhejiang University, Zhejiang, China. Electronic address: shenweiliang365@163.com.
  • Ouyang HW Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Zhejiang 310000, China; Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, School of Medicine, Zhejiang University, Zhejiang 310009, China; Department of Sports Medicine, School of Medicine, Zhejiang University, Zhejiang 310000, China; China Orthopaedic Regenerative Medicine (CORMed), Hangzhou, China.
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  • 2017-01-18
Published in:
  • Acta biomaterialia. - 2017
Macroporous three-dimensional aligned collagen/silk scaffold
Regeneration
Rotator cuff
Tissue engineering
Animals
Biomechanical Phenomena
Bombyx
Cell Proliferation
Disease Models, Animal
Female
Fibrillar Collagens
Gene Expression Regulation
Implants, Experimental
Porosity
Rabbits
Real-Time Polymerase Chain Reaction
Regeneration
Rotator Cuff
Silk
Sus scrofa
Tissue Scaffolds
English Rotator cuff tear is one of the most common types of shoulder injuries, often resulting in pain and physical debilitation. Allogeneic tendon-derived decellularized matrices do not have appropriate pore size and porosity to facilitate cell infiltration, while commercially-available synthetic scaffolds are often inadequate at inducing tenogenic differentiation. The aim of this study is to develop an advanced 3D aligned collagen/silk scaffold (ACS) and investigate its efficacy in a rabbit massive rotator cuff tear model. ACS has similar 3D alignment of collagen fibers as natural tendon with superior mechanical characteristics. Based on ectopic transplantation studies, the optimal collagen concentration (10mg/ml), pore diameter (108.43±7.25μm) and porosity (97.94±0.08%) required for sustaining a stable macro-structure conducive for cellular infiltration was determined. Within in vitro culture, tendon stem/progenitor cells (TSPCs) displayed spindle-shaped morphology, and were well-aligned on ACS as early as 24h. TSPCs formed intercellular contacts and deposited extracellular matrix after 7days. With the in vivo rotator cuff repair model, the regenerative tendon of the ACS group displayed more conspicuous native microstructures with larger diameter collagen fibrils (48.72±3.75 vs. 44.26±5.03nm) that had better alignment and mechanical properties (139.85±49.36vs. 99.09±33.98N) at 12weeks post-implantation. In conclusion, these findings demonstrate the positive efficacy of the macroporous 3D aligned scaffold in facilitating rotator cuff tendon regeneration, and its practical applications for rotator cuff tendon tissue engineering.


STATEMENT OF SIGNIFICANCE
Massive rotator cuff tear is one of the most common shoulder injuries, and poses a formidable clinical challenge to the orthopedic surgeon. Tissue engineering of tendon can potentially overcome the problem. However, more efficacious scaffolds with good biocompatibility, appropriate pore size, favorable inductivity and sufficient mechanical strength for repairing massive rotator cuff tendon injuries need to be developed. In this study, we developed a novel macroporous 3D aligned collagen/silk scaffold, and demonstrated that this novel scaffold enhanced the efficacy of rotator cuff tendon regeneration by inducing aligned supracellular structures similar to natural tendon, which in turn enhanced cellular infiltration and tenogenic differentiation of stem/progenitor cells from both the tendon itself and surrounding tissues. Hence, it can potentially be a clinically useful application for tendon tissue engineering.
Language
  • English
Open access status
green
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/105056
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