Back
Chemical and Biological Dynamics Using Droplet-Based Microfluidics.
Journal article

Chemical and Biological Dynamics Using Droplet-Based Microfluidics.

  • Dressler OJ Department of Chemistry and Applied Biosciences, ETH Zürich, CH-8093 Zürich, Switzerland; email: andrew.demello@chem.ethz.ch.
  • Casadevall I Solvas X Department of Chemistry and Applied Biosciences, ETH Zürich, CH-8093 Zürich, Switzerland; email: andrew.demello@chem.ethz.ch.
  • deMello AJ Department of Chemistry and Applied Biosciences, ETH Zürich, CH-8093 Zürich, Switzerland; email: andrew.demello@chem.ethz.ch.
  • 2017-04-05
Published in:
  • Annual review of analytical chemistry (Palo Alto, Calif.). - 2017
droplets
high-throughput experimentation
kinetics
microfluidics
optical detection
Biological Assay
Enzymes
Kinetics
Microfluidics
Nucleic Acids
Oils
Spectrometry, Fluorescence
Surface-Active Agents
English Recent years have witnessed an increased use of droplet-based microfluidic techniques in a wide variety of chemical and biological assays. Nevertheless, obtaining dynamic data from these platforms has remained challenging, as this often requires reading the same droplets (possibly thousands of them) multiple times over a wide range of intervals (from milliseconds to hours). In this review, we introduce the elemental techniques for the formation and manipulation of microfluidic droplets, together with the most recent developments in these areas. We then discuss a wide range of analytical methods that have been successfully adapted for analyte detection in droplets. Finally, we highlight a diversity of studies where droplet-based microfluidic strategies have enabled the characterization of dynamic systems that would otherwise have remained unexplorable.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/104355
Statistics
Document views: 9 File downloads: