Back
A review on recent progress in the detection methods and prevalence of human enteric viruses in water.
Journal article

A review on recent progress in the detection methods and prevalence of human enteric viruses in water.

  • Haramoto E Interdisciplinary Center for River Basin Environment, Graduate Faculty of Interdisciplinary Research, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan. Electronic address: eharamoto@yamanashi.ac.jp.
  • Kitajima M Division of Environmental Engineering, Faculty of Engineering, Hokkaido University, North 13 West 8, Kita-ku, Sapporo, Hokkaido 060-8628, Japan. Electronic address: mkitajima@eng.hokudai.ac.jp.
  • Hata A Integrated Research System for Sustainability Science, Institutes for Advanced Study, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8654, Japan. Electronic address: hata@ir3s.u-tokyo.ac.jp.
  • Torrey JR School of Architecture, Civil and Environmental Engineering, École Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland. Electronic address: jason.torrey@epfl.ch.
  • Masago Y Institute for the Advanced Study of Sustainability, United Nations University, 5-53-70 Jingumae, Shibuya-ku, Tokyo 150-8925, Japan. Electronic address: masago@unu.edu.
  • Sano D Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, Aoba 6-6-06, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan. Electronic address: daisuke.sano.e1@tohoku.ac.jp.
  • Katayama H Department of Urban Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan; Vietnam Japan University, Luu Huu Phuoc Road, My Dinh 1 Ward, Nam Tu Liem District, Ha Noi, Vietnam. Electronic address: katayama@env.t.u-tokyo.ac.jp.
Show more…
  • 2018-02-23
Published in:
  • Water research. - 2018
Human enteric viruses
Process control
Viral metagenomics
Virus detection method
Enterovirus
Enterovirus Infections
Feces
Fresh Water
Genome, Viral
Humans
Polymerase Chain Reaction
English Waterborne human enteric viruses, such as noroviruses and adenoviruses, are excreted in the feces of infected individuals and transmitted via the fecal-oral route including contaminated food and water. Since viruses are normally present at low concentrations in aquatic environments, they should be concentrated into smaller volumes prior to downstream molecular biological applications, such as quantitative polymerase chain reaction (qPCR). This review describes recent progress made in the development of concentration and detection methods of human enteric viruses in water, and discusses their applications for providing a better understanding of the prevalence of the viruses in various types of water worldwide. Maximum concentrations of human enteric viruses in water that have been reported in previous studies are summarized to assess viral abundances in aquatic environments. Some descriptions are also available on recent applications of sequencing analyses used to determine the genetic diversity of viral genomes in water samples, including those of novel viruses. Furthermore, the importance and significance of utilizing appropriate process controls during viral analyses are discussed, and three types of process controls are considered: whole process controls, molecular process controls, and (reverse transcription (RT)-)qPCR controls. Although no standards have been established for acceptable values of virus recovery and/or extraction-(RT-)qPCR efficiency, use of at least one of these appropriate control types is highly recommended for more accurate interpretation of observed data.
Language
  • English
Open access status
hybrid
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/102657
Statistics
Document views: 3 File downloads: