Journal article
Hectd1 is essential for embryogenesis in mice.
- D'Alonzo D Department of Biomedicine, University Hospital, University of Basel, Hebelstrasse 20, CH-4031, Basel, Switzerland.
- Emch FH Clinic of Gynecological Endocrinology and Reproductive Medicine, University Hospital, University of Basel, Spitalstrasse 21, CH-4031, Basel, Switzerland.
- Shen X Clinic of Gynecological Endocrinology and Reproductive Medicine, University Hospital, University of Basel, Spitalstrasse 21, CH-4031, Basel, Switzerland; Chongqing Reproductive and Genetics Institute, 64 Jing Tang ST, Yu Zhong District, Chongqing, 400013, PR China.
- Bruder E Institute of Pathology, University Hospital, University of Basel, Schönbeinstrasse 40, CH-4031, Basel, Switzerland.
- De Geyter C Department of Biomedicine, University Hospital, University of Basel, Hebelstrasse 20, CH-4031, Basel, Switzerland; Clinic of Gynecological Endocrinology and Reproductive Medicine, University Hospital, University of Basel, Spitalstrasse 21, CH-4031, Basel, Switzerland.
- Zhang H Department of Biomedicine, University Hospital, University of Basel, Hebelstrasse 20, CH-4031, Basel, Switzerland. Electronic address: hong.zhang@usb.ch.
- 2019-07-14
Published in:
- Gene expression patterns : GEP. - 2019
Gene expression
Gene trap
Hectd1
Mutant mouse
X-gal
Animals
Embryonic Development
Female
Gene Expression
Gene Expression Regulation, Developmental
Humans
Mice
Mice, Transgenic
Placenta
Placentation
Pregnancy
Ubiquitin-Protein Ligases
English
Many aspects of the functional role of the E3 ubiquitin ligase Hectd1 in embryogenesis and in cell biology still remain to be elucidated. In order to contribute to this task we now report the generation of a new transgenic mouse model for Hectd1 using the gene trap strategy. The HECT domain deletion mutant mouse was created by inserting a β-geo cassette into the Hectd1 locus. Mice homozygous for Hectd1-mutant showed early embryonic lethality with abnormal placental development and defective of neural tube closure resulting in exencephaly. The thickness of the placenta of both Hectd1-mutant homozygous and heterozygous mice was distinctly thinner than that of wildtype mice, the difference being most pronounced in the labyrinth layer of the placenta. We also addressed the temporal and spatial expression profiles of Hectd1 in adult tissues by X-gal staining. Hectd1 expression was detected in specific cell populations of most but not all tissues of the adult organism. Furthermore, the expression of Hectd1 was regulated by insulin and by both heat and hypoxia. Thus, our studies reveal that Hectd1 is indispensable for normal embryogenesis and fetal survival. The generation of this new Hectd1 mutant mouse model provides ample opportunities to study the function of Hectd1 in mammalian cells in detail.
- Language
-
- English
- Open access status
- closed
- Identifiers
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- DOI 10.1016/j.gep.2019.119064
- PMID 31301385
- Persistent URL
- https://folia.unifr.ch/global/documents/102131
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