Back
Full-text
Journal article

Differential transcriptional responses to Ebola and Marburg virus infection in bat and human cells.

  • Hölzer M RNA Bioinformatics and High Throughput Analysis, Faculty of Mathematics and Computer Science, Friedrich Schiller University Jena, Leutragraben 1, 07743, Jena, Germany.
  • Krähling V Institute of Virology, Philipps University Marburg, Hans-Meerwein-Str. 2, 35043 Marburg, Germany.
  • Amman F Bioinformatics Group, Department of Computer Science, University of Leipzig, Härtelstraße 16-18, 04107, Leipzig, Germany.
  • Barth E RNA Bioinformatics and High Throughput Analysis, Faculty of Mathematics and Computer Science, Friedrich Schiller University Jena, Leutragraben 1, 07743, Jena, Germany.
  • Bernhart SH Transcriptome Bioinformatics, Junior Research Group, Leipzig Research Center for Civilization Diseases, University of Leipzig, Härtelstraße 16-18, 04107, Leipzig, Germany.
  • Carmelo VA Center for non-coding RNA in Technology and Health, University of Copenhagen, Grønnegårdsvej 3, 1870, Frederiksberg C, Denmark.
  • Collatz M RNA Bioinformatics and High Throughput Analysis, Faculty of Mathematics and Computer Science, Friedrich Schiller University Jena, Leutragraben 1, 07743, Jena, Germany.
  • Doose G Transcriptome Bioinformatics, Junior Research Group, Leipzig Research Center for Civilization Diseases, University of Leipzig, Härtelstraße 16-18, 04107, Leipzig, Germany.
  • Eggenhofer F Theoretical Biochemistry Group, Institute of Theoretical Chemistry, University of Vienna, Währingerstraße 17, 1090, Vienna, Austria.
  • Ewald J Research Group Theoretical Systems Biology, Department of Bioinformatics, Friedrich Schiller University Jena, Ernst-Abbe-Platz 2, 07743, Jena, Germany.
  • Fallmann J Bioinformatics Group, Department of Computer Science, University of Leipzig, Härtelstraße 16-18, 04107, Leipzig, Germany.
  • Feldhahn LM Institute of Computer Science, Martin-Luther University Halle-Wittenberg, Von-Seckendorff-Platz 1, 06120, Halle/Saale, Germany.
  • Fricke M RNA Bioinformatics and High Throughput Analysis, Faculty of Mathematics and Computer Science, Friedrich Schiller University Jena, Leutragraben 1, 07743, Jena, Germany.
  • Gebauer J Research Group Theoretical Systems Biology, Department of Bioinformatics, Friedrich Schiller University Jena, Ernst-Abbe-Platz 2, 07743, Jena, Germany.
  • Gruber AJ Biozentrum, University of Basel, Klingelbergstraße 50/70, CH-4056, Basel, Switzerland.
  • Hufsky F RNA Bioinformatics and High Throughput Analysis, Faculty of Mathematics and Computer Science, Friedrich Schiller University Jena, Leutragraben 1, 07743, Jena, Germany.
  • Indrischek H Bioinformatics Group, Department of Computer Science, University of Leipzig, Härtelstraße 16-18, 04107, Leipzig, Germany.
  • Kanton S TFome Research Group, Bioinformatics Group, Interdisciplinary Center of Bioinformatics, Department of Computer Science, University of Leipzig, Härtelstraße 16-18, 04107, Leipzig, Germany.
  • Linde J Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute (HKI), Systems Biology and Bioinformatics, Beutenbergstraße 11a, 07745, Jena, Germany.
  • Mostajo N RNA Bioinformatics and High Throughput Analysis, Faculty of Mathematics and Computer Science, Friedrich Schiller University Jena, Leutragraben 1, 07743, Jena, Germany.
  • Ochsenreiter R Theoretical Biochemistry Group, Institute of Theoretical Chemistry, University of Vienna, Währingerstraße 17, 1090, Vienna, Austria.
  • Riege K RNA Bioinformatics and High Throughput Analysis, Faculty of Mathematics and Computer Science, Friedrich Schiller University Jena, Leutragraben 1, 07743, Jena, Germany.
  • Rivarola-Duarte L Bioinformatics Group, Department of Computer Science, University of Leipzig, Härtelstraße 16-18, 04107, Leipzig, Germany.
  • Sahyoun AH RNA Bioinformatics and High Throughput Analysis, Faculty of Mathematics and Computer Science, Friedrich Schiller University Jena, Leutragraben 1, 07743, Jena, Germany.
  • Saunders SJ Bioinformatics Group, Department of Computer Science, University of Freiburg, Georges-Köhler-Allee 106, 79110, Freiburg, Germany.
  • Seemann SE Center for non-coding RNA in Technology and Health, University of Copenhagen, Grønnegårdsvej 3, 1870, Frederiksberg C, Denmark.
  • Tanzer A Theoretical Biochemistry Group, Institute of Theoretical Chemistry, University of Vienna, Währingerstraße 17, 1090, Vienna, Austria.
  • Vogel B RNA Bioinformatics and High Throughput Analysis, Faculty of Mathematics and Computer Science, Friedrich Schiller University Jena, Leutragraben 1, 07743, Jena, Germany.
  • Wehner S RNA Bioinformatics and High Throughput Analysis, Faculty of Mathematics and Computer Science, Friedrich Schiller University Jena, Leutragraben 1, 07743, Jena, Germany.
  • Wolfinger MT Theoretical Biochemistry Group, Institute of Theoretical Chemistry, University of Vienna, Währingerstraße 17, 1090, Vienna, Austria.
  • Backofen R Center for non-coding RNA in Technology and Health, University of Copenhagen, Grønnegårdsvej 3, 1870, Frederiksberg C, Denmark.
  • Gorodkin J Center for non-coding RNA in Technology and Health, University of Copenhagen, Grønnegårdsvej 3, 1870, Frederiksberg C, Denmark.
  • Grosse I Institute of Computer Science, Martin-Luther University Halle-Wittenberg, Von-Seckendorff-Platz 1, 06120, Halle/Saale, Germany.
  • Hofacker I Center for non-coding RNA in Technology and Health, University of Copenhagen, Grønnegårdsvej 3, 1870, Frederiksberg C, Denmark.
  • Hoffmann S Transcriptome Bioinformatics, Junior Research Group, Leipzig Research Center for Civilization Diseases, University of Leipzig, Härtelstraße 16-18, 04107, Leipzig, Germany.
  • Kaleta C Research Group Medical Systems Biology, Institute for Experimental Medicine, Christian-Albrechts-University Kiel, Brunswiker Str. 10, 24105, Kiel, Germany.
  • Stadler PF Bioinformatics Group, Department of Computer Science, University of Leipzig, Härtelstraße 16-18, 04107, Leipzig, Germany.
  • Becker S Institute of Virology, Philipps University Marburg, Hans-Meerwein-Str. 2, 35043 Marburg, Germany.
  • Marz M RNA Bioinformatics and High Throughput Analysis, Faculty of Mathematics and Computer Science, Friedrich Schiller University Jena, Leutragraben 1, 07743, Jena, Germany.
Show more…
  • 2016-10-08
Published in:
  • Scientific reports. - 2016
Animals
Cell Line, Tumor
Chiroptera
Ebolavirus
Gene Expression Regulation
Hemorrhagic Fever, Ebola
Humans
Marburg Virus Disease
Marburgvirus
Signal Transduction
Transcription, Genetic
English The unprecedented outbreak of Ebola in West Africa resulted in over 28,000 cases and 11,000 deaths, underlining the need for a better understanding of the biology of this highly pathogenic virus to develop specific counter strategies. Two filoviruses, the Ebola and Marburg viruses, result in a severe and often fatal infection in humans. However, bats are natural hosts and survive filovirus infections without obvious symptoms. The molecular basis of this striking difference in the response to filovirus infections is not well understood. We report a systematic overview of differentially expressed genes, activity motifs and pathways in human and bat cells infected with the Ebola and Marburg viruses, and we demonstrate that the replication of filoviruses is more rapid in human cells than in bat cells. We also found that the most strongly regulated genes upon filovirus infection are chemokine ligands and transcription factors. We observed a strong induction of the JAK/STAT pathway, of several genes encoding inhibitors of MAP kinases (DUSP genes) and of PPP1R15A, which is involved in ER stress-induced cell death. We used comparative transcriptomics to provide a data resource that can be used to identify cellular responses that might allow bats to survive filovirus infections.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/270458
Statistics
Document views: 10 File downloads:
  • fulltext.pdf: 0