Back
Full-text
Journal article

ACE2 localizes to the respiratory cilia and is not increased by ACE inhibitors or ARBs.

  • Lee IT Department of Pathology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
  • Nakayama T Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA.
  • Wu CT Baxter Laboratory, Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CA, USA.
  • Goltsev Y Department of Pathology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
  • Jiang S Department of Pathology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
  • Gall PA Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA.
  • Liao CK Department of Otolaryngology, National Taiwan University Hospital, Taipei, Taiwan.
  • Shih LC Department of Otorhinolaryngology, China Medical University Hospital, Taichung, Taiwan.
  • Schürch CM Department of Pathology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
  • McIlwain DR Department of Pathology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
  • Chu P Department of Pathology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
  • Borchard NA Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA.
  • Zarabanda D Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA.
  • Dholakia SS Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA.
  • Yang A Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA.
  • Kim D Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA.
  • Chen H Department of Pathology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
  • Kanie T Baxter Laboratory, Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CA, USA.
  • Lin CD Department of Otorhinolaryngology, China Medical University Hospital, Taichung, Taiwan.
  • Tsai MH Department of Otorhinolaryngology, China Medical University Hospital, Taichung, Taiwan.
  • Phillips KM Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA.
  • Kim R Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA.
  • Overdevest JB Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA.
  • Tyler MA Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA.
  • Yan CH Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA.
  • Lin CF Department of Otolaryngology, National Taiwan University Hospital, Taipei, Taiwan.
  • Lin YT Department of Otolaryngology, National Taiwan University Hospital, Taipei, Taiwan.
  • Bau DT Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan.
  • Tsay GJ School of Medicine, China Medical University, Taichung, Taiwan.
  • Patel ZM Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA.
  • Tsou YA Department of Otorhinolaryngology, China Medical University Hospital, Taichung, Taiwan.
  • Tzankov A Pathology, Institute of Medical Genetics and Pathology, University Hospital Basel, University of Basel, Basel, Switzerland.
  • Matter MS Pathology, Institute of Medical Genetics and Pathology, University Hospital Basel, University of Basel, Basel, Switzerland.
  • Tai CJ Department of Otorhinolaryngology, China Medical University Hospital, Taichung, Taiwan.
  • Yeh TH Department of Otolaryngology, National Taiwan University Hospital, Taipei, Taiwan.
  • Hwang PH Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA.
  • Nolan GP Department of Pathology, Stanford University School of Medicine, Stanford, CA, 94305, USA. gnolan@stanford.edu.
  • Nayak JV Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA.
  • Jackson PK Department of Pathology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Show more…
  • 2020-10-29
Published in:
  • Nature communications. - 2020
Age Factors
Angiotensin Receptor Antagonists
Angiotensin-Converting Enzyme Inhibitors
Cilia
Coronavirus Infections
Endothelial Cells
Gene Expression
Goblet Cells
Humans
Lung
Pandemics
Peptidyl-Dipeptidase A
Pneumonia, Viral
Respiratory System
Sex Factors
Sinusitis
Smoking
English The coronavirus SARS-CoV-2 is the causative agent of the ongoing severe acute respiratory disease pandemic COVID-19. Tissue and cellular tropism is one key to understanding the pathogenesis of SARS-CoV-2. We investigate the expression and subcellular localization of the SARS-CoV-2 receptor, angiotensin-converting enzyme 2 (ACE2), within the upper (nasal) and lower (pulmonary) respiratory tracts of human donors using a diverse panel of banked tissues. Here, we report our discovery that the ACE2 receptor protein robustly localizes within the motile cilia of airway epithelial cells, which likely represents the initial or early subcellular site of SARS-CoV-2 viral entry during host respiratory transmission. We further determine whether ciliary ACE2 expression in the upper airway is influenced by patient demographics, clinical characteristics, comorbidities, or medication use, and show the first mechanistic evidence that the use of angiotensin-converting enzyme inhibitors (ACEI) or angiotensin II receptor blockers (ARBs) does not increase susceptibility to SARS-CoV-2 infection through enhancing the expression of ciliary ACE2 receptor. These findings are crucial to our understanding of the transmission of SARS-CoV-2 for prevention and control of this virulent pathogen.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/189254
Statistics
Document views: 45 File downloads:
  • Full-text: 0