Gene Brief description References Accession numbers Species Most probable homolog in D. magna Locus tag in D. magna Contrast MP vs. NMP MP-MF vs. NMP-MF MP vs. MP-MF NMP vs. NMP-MF log2FC (MP relative to NMP) p-adj log2FC (MP relative to NMP) p-adj log2FC (Control relative to MF) p-adj Log2FC (Control relative to MF) p-adj "Csd, Complementary sex determiner-like protein" The complementary sex determiner was considered to have arisen from duplication of the fem gene and has already been looked for in D. magna: it corresponds with fem to a unique transformer gene. "Schmieder et al., 2012; Kato et al., 2011" LRGB01000868 Genomic DNA Translation: KZS15621.1 D. magna NA APZ42_018795 0.1105 0.7073 0.0011 0.9973 0.0804 0.6068 -0.0375 0.8895 "Dsx1, doublesex 1 alpha and beta" Doublesex is the terminal molecule involved in male-specific trait expression in D. magna. "Nong et al., 2017; Kato et al., 2011" "AB569296 mRNA Translation: BAJ78307.1 AB569297 mRNA Translation: BAJ78308.1" D. magna NA APZ42_027481 -1.2510 0.0038 -1.2199 0.0002 0.1132 0.6436 0.2560 0.0469 "Dsx2, doublesex 2" Doublesex is the terminal molecule involved in male-specific trait expression in D. magna. "Nong et al., 2017; Kato et al., 2011" AB569298 mRNA Translation: BAJ78309.1 D. magna NA APZ42_027480 0.0636 0.9853 -0.1185 0.9452 0.3575 0.1191 0.1653 0.4301 cytochrome b5 It was also found DE in response to MF in the study from Toyota et al. 2017. "Toyota et al., 2017" LRGB01002384 Genomic DNA Translation: KZS07998.1 D. magna NA APZ42_028389 0.0795 0.8973 -0.0153 0.9730 0.0415 0.8175 -0.0646 0.7147 DMRT11E They are the first DM domain genes identified in crustaceans and DM-domains of Daphnia are well conserved. "Kato et al., 2008" AB361069 mRNA Translation: BAG12871.1 D. magna NA APZ42_029166 -0.1998 0.9230 0.1293 0.8749 0.0798 0.8554 0.4239 0.0398 DMRT93B They are the first DM domain genes identified in crustaceans and DM-domains of Daphnia are well conserved. "Kato et al., 2008" AB361070 mRNA Translation: BAG12872.1 D. magna NA APZ42_030657 -0.1571 0.9660 0.3695 NA 0.0627 NA 0.4372 NA DMRT99B They are the first DM domain genes identified in crustaceans and DM-domains of Daphnia are well conserved. "Kato et al., 2008" AB361071 mRNA Translation: BAG12873.1 D. magna NA APZ42_014958 -0.8994 0.1738 -0.4554 0.5570 -0.2308 0.6252 0.2596 0.4740 "Fru, Sex determination protein fruitless" "In Drosophila, its sex-specific alternative splicing forms of the fruitless gene is induced by the transformer genes." "Heinrichs et al., 1998" GDIP01016924 Transcribed RNA Translation: JAM86791.1 from Orsini et al 2016 D. magna NA APZ42_029771 0.1819 0.8407 0.1111 0.8274 -0.0425 0.8524 -0.1237 0.3054 FTZ-F1 The Fushi tarazu transcription factor-1 has been found in D. magna in two splicing variants that are over-expressed in males vs. Females. "Mohamad Ishak et al., 2016," "LC105700, GenBank: BAU20371.1" D. magna NA APZ42_031882 -0.3180 0.9182 -0.9910 0.2560 1.2691 0.0000 0.6156 0.1221 MET "The complex MET-SRC: Methropene tolerant protein - steroid receptor coactivator dimer, both member of the bHLH-PAS family of transcription factors) is a direct receptor of methylfarnesoate." "LeBlanc et al., 2013 LeBlanc and Medlock, 2015" AB698069 mRNA Translation: BAM83855.1 D. magna NA APZ42_028786 -0.0477 0.9815 0.0382 0.9636 0.2471 0.2315 0.3211 0.2986 SRC "The complex MET-SRC: Methropene tolerant protein - steroid receptor coactivator dimer, both member of the bHLH-PAS family of transcription factors) is a direct receptor of methylfarnesoate." "LeBlanc et al., 2013 LeBlanc and Medlock, 2015" AB698070 mRNA Translation: BAM83856.1 D. magna NA APZ42_031134 -0.4239 0.8624 -0.3523 0.7867 -0.4396 0.4062 -0.2129 0.6406 "NR2, NMDA receptor 2" "Methyl farnesoate receptors as RXR, NR2B are protein receptor of methylfarnesoate, they are mediator of MF-signaling; the N-methyl-D-aspartic acid receptor is regulated upstream the MF for male production in D. pulex." "LeBlanc et al., 2013 LeBlanc and Medlock, 2015" LRGB01002849 Genomic DNA Translation: KZS06096.1 D. magna NA APZ42_030566 1.1439 0.3054 -0.2138 0.9233 1.0341 0.0698 -0.6308 0.3688 Retinoic acid receptor RXR-alpha-A "Methyl farnesoate receptors as RXR, NR2B are protein receptor of methylfarnesoate, they are mediator of MF-signaling." "LeBlanc et al., 2013 LeBlanc and Medlock, 2015, Wang YH & LeBlanc GA (2009)" LRGB01002443 Genomic DNA Translation: KZS07689.1 D. magna NA APZ42_028519 0.1718 0.5243 0.0004 0.9991 0.2224 0.0962 0.0427 0.8822 Sex lethal Gene whose its differential expression initiates the sex determining cascade (acting on the RNA of the transformer gene) in insects. "LeBlanc and Medlock, 2015" LRGB01002864 Genomic DNA Translation: KZS05769.1 D. magna NA APZ42_031015 0.0441 0.9451 -0.1246 0.8437 0.2290 0.2583 0.0393 0.8920 SOX-10 "These genes are part of the ""usual suspects""." "Herpin and Schartl, 2015." LRGB01002066 Genomic DNA Translation: KZS09457.1 D. magna NA APZ42_026255 0.0354 0.9888 0.1431 0.9020 -0.5524 0.1142 -0.4638 0.1482 SOX-14 "These genes are part of the ""usual suspects""." "Herpin and Schartl, 2015." GDIQ01019318 Transcribed RNA Translation: JAN75419.1 D. magna NA APZ42_026252 0.5049 0.7973 0.4686 0.6576 -0.2441 0.7103 -0.2912 0.7155 SOX-2 "These genes are part of the ""usual suspects""." "Herpin and Schartl, 2015." LRGB01002993 Genomic DNA Translation: KZS05152.1 D. magna NA APZ42_031711 -0.6263 0.7479 0.6740 0.4937 -0.5627 NA 0.5648 0.3981 SOX-4 "These genes are part of the ""usual suspects""." "Herpin and Schartl, 2015." GDIP01023222 Transcribed RNA Translation: JAM80493.1 D. magna NA APZ42_016821 -0.3298 0.8796 -0.2959 0.8258 0.3192 0.6018 0.4516 0.1511 SOX-6 "These genes are part of the ""usual suspects""." "Herpin and Schartl, 2015." GDIQ01005528 Transcribed RNA Translation: JAN89209.1 D. magna NA APZ42_021014 0.1452 0.9467 0.1603 0.8891 0.0627 0.8342 0.0969 0.8506 SOX-7 "These genes are part of the ""usual suspects""." "Herpin and Schartl, 2015." GDIP01165431 Transcribed RNA Translation: JAJ57971.1 D. magna NA APZ42_019202 0.7381 0.6631 1.0702 0.1744 -0.5438 0.1339 -0.2368 0.3730 SOX-9 "These genes are part of the ""usual suspects""." "Herpin and Schartl, 2015." GDIP01014572 Transcribed RNA Translation: JAM89143.1 D. magna NA APZ42_029254 0.1632 0.9599 0.5615 0.6529 -0.8366 0.1296 -0.5148 0.3391 "Tra, Transformer" "The transformer gene is a doublesex splicing factor regulator, already found in a single copy in D. magna." "Verhulst, van de Zande and Beukeboom, 2010; Kato et al., 2010" "AB523902.1 transalation, BAI66432.1""" D. magna NA APZ42_018795 0.1105 0.7073 0.0011 0.9973 0.0804 0.6068 -0.0375 0.8895 "Vg, Vitellogenin" They are vitellogenin genes whose the expression can variate through the action of juvenile hormone (as methylfarnesoate). "Tokishita et al., 2006; Toyota et al., 2016" GDIP01034641 Transcribed RNA Translation: JAM69074.1 D. magna NA APZ42_004153 0.6049 0.7833 -0.0848 0.9600 0.8394 0.0384 0.2590 0.6270 "Vg, Vitellogenin" They are vitellogenin genes whose the expression can variate through the action of juvenile hormone (as methylfarnesoate). "Tokishita et al., 2006; Toyota et al., 2016" GDIP01023156 Transcribed RNA Translation: JAM80559.1 D. magna NA APZ42_025127 0.2668 0.9212 0.4848 0.3776 -0.4277 0.4246 -0.1631 0.7277 "Vg, Vitellogenin" They are vitellogenin genes whose the expression can variate through the action of juvenile hormone (as methylfarnesoate). "Tokishita et al., 2006; Toyota et al., 2016" LRGB01015451 Genomic DNA Translation: KZR98887.1 D. magna NA APZ42_005485 0.1922 0.9451 0.5193 0.6049 -0.5175 0.1094 -0.0895 0.8966 "Vg, Vitellogenin" They are vitellogenin genes whose the expression can variate through the action of juvenile hormone (as methylfarnesoate). "Tokishita et al., 2006; Toyota et al., 2016" AB252738 mRNA Translation: BAE94324.1 D. magna NA APZ42_001431 0.0306 0.9930 0.5591 0.6024 0.0920 0.9124 0.6223 0.0048 "Vri, Vrille" The bZIP transcript factor binding site is necessary and sufficient for Dsx1 activation with an up-regulation and finally male induction in D. magna. However no male specific expression of Vri was found in late embryo only in early gastrula. "Mohamad Ishak et al., 2017" LC230164 mRNA Translation: BAX77792.1 D. magna NA APZ42_033931 0.1801 0.9326 0.4407 0.4724 -1.0241 0.0010 -0.8768 0.0000 "Fem, Feminizer" "The feminizer micro RNA gene, fisrt described in Apis mellifera, is expressed on W only and regulate dsx female isoform." "Hasselmann et al., 2008" EU101388 mRNA Translation: ABU68668.1;GeneID:724970 Apis melifera KZS15621.1Complementary sex determiner-like protein [Daphnia magna] APZ42_018795 0.1105 0.7073 0.0011 0.9973 0.0804 0.6068 -0.0375 0.8895 "Fem, Feminizer" "The feminizer micro RNA gene, fisrt described in Apis mellifera, is expressed on W only and regulate dsx female isoform." "Hasselmann et al., 2008" XM_017195636; transalation: XP_017051125.1;GeneID:108094839 Drosophila ficusphila KZS14242.1Protein fem-1 B [Daphnia magna] APZ42_020448 0.0895 0.8592 -0.0999 0.7762 0.1368 0.4243 -0.0599 0.8000 "Chk1, Chk kinase 1" Genes encoding chk1 (checkpoint kinase 1) may be involved in sexual differentiation in D. pulex and was found to be DE in response to MF in D. magna. "Guo et al., 2015; Toyota et al., 2017; Kong, L., et al. 2016." KJ735446 mRNA Translation: AJP08956.1 Daphnia carinata KZS13438.1Serine/threonine-protein kinase GRP [Daphnia magna] APZ42_021409 -0.0731 0.9467 -0.1188 0.8561 0.0823 0.7339 0.0171 0.9507 "Trh, Trachealess " Encodes a bHLH-PAS clock protein. bHLH-PAS proteins has been found in Drosophila to be involved in sex determination. "Caudy et al., 1988; Murre et al., 1994; Zelzer, Wappner and Shilo, 1995" U33427.1 Translation: AAA96257.1 Drosophila melanogaster KZS17728.1putative Protein trachealess [Daphnia magna] APZ42_016247 0.3078 0.8824 0.0656 0.9578 -0.2962 0.5300 -0.5281 0.0603 "Cyc, Cycle" Cycle is also a bHLH-PAS clock protein. bHLH-PAS proteins has been found in Drosophila to be involved in sex determination. "Caudy et al., 1988; Murre et al., 1994; Zelzer, Wappner and Shilo, 1996" "AF065473, translation: AAC39124.1" Drosophila melanogaster KZS03618.1Aryl hydrocarbon receptor nuclear translocator protein 1 [Daphnia magna] APZ42_033598 -0.0208 0.9937 -0.1038 0.9089 0.4406 0.0962 0.3166 0.3760 "Da, Daughterless" Daughterless is a bHLH-PAS clock protein. bHLH-PAS proteins has been found in Drosophila to be involved in sex determination. "Caudy et al., 1988; Murre et al., 1994; Zelzer, Wappner and Shilo, 1997" " J03148 mRNA Translation: AAA28442.1" Drosophila melanogaster KZS11828.1putative Daughterless protein [Daphnia magna] APZ42_023386 -0.4420 0.7847 0.3429 0.7422 -0.2657 0.7041 0.4148 0.4507 "ACE, Angiotensin-converting enzyme" "The “Angiotensin-converting enzyme” was found DE (FC > 1.5, p < 0.1) between ovaries of MP vs. MP-MF individuals in Toyota et al. (2017) microarray analysis." Toyota et al. (2017) GDIQ01083183 Transcribed RNA Translation: JAN11554.1 Daphnia magna NA APZ42_013867 0.7375 0.0001 0.4214 0.4365 -0.1099 0.7771 -0.4462 0.0015 "FAMeT, FAMT, Farnesoic acid o- methyltransferase" Methyl farnesoate is produced by the enzymes farnesoic acid o- methyltransferase (FAMT) and juvenile hormone acid o-methyltransferase (JHAMT). "Camp et al. (2019); (Toyota et al., 2015a)" NCBI_GNO_180083 Daphnia pulex KZS18550.1 C3 and PZP alpha-2-macroglobulin domain-containing protein 8 [Daphnia magna] APZ42_015076 0.0901 0.9461 0.0480 0.9619 0.1417 0.1202 0.1011 0.7538 "JHAMT, Juvenile hormone acid o-methyltransferase" Methyl farnesoate is produced by the enzymes farnesoic acid o- methyltransferase (FAMT) and juvenile hormone acid o-methyltransferase (JHAMT). "Camp et al. (2019); (Xie et al., 2016) (Miyakawa et al., 2010)" NCBI_GNO_2422013; AB222845 Daphnia pulex Not found Gene 8960 Candidate gene identified in D. pulex to be responsible for the NMP phenotype. Ye et al. Under review Supplementary data from Ye et al. (2017) Daphnia pulex KZS13712.1 Uncharacterized protein [Daphnia magna] APZ42_021088 1.2842 0.1342 0.3213 0.8264 -0.6262 0.3532 -1.0526 0.0437