Sequencing primers for dgat1 carried by the eve4 mutant		
			
	Primers	5'-3' sequence	
	DGAT_SP6_F1	GATTTAGGTGACACTATAGGGAACCAGACACGAATCCCA	SP6
	DGAT_T7_R1	TAATACGACTCACTATAGGCTGGATACAAAACCTCTGTC	T7
	DGAT_SP6_F2	GATTTAGGTGACACTATAGTCGATCTTTCCTTTGGCTGC	SP6
	DGAT_T7_R2	TAATACGACTCACTATAGGCCATACCATATTCCACATTC	T7
	DGAT_SP6_F3	GATTTAGGTGACACTATAGCCTTTGGTATGCTGTGATCC	SP6
	DGAT_T7_R3	TAATACGACTCACTATAGGGTTTTAGCATCAGAACCACC	T7
			
	To facilitate Sanger sequencing, forward and reverse primers included the SP6 and T7 promoter sequences, respectively (underlined).		
			
			
			
				
	Genotyping primers.			
				
	Allele	Primers	5'-3' sequence	Band size (bp)
	GGR	GGR_F	GCTGAGAAGAGTGGAGCCAC	
		GGR_R	CAATGGTGTTCACAGCCAAC	1071
				
	ggr-1	GGR_F	GCTGAGAAGAGTGGAGCCAC	
		LBb1.3	ATTTTGCCGATTTCGGAAC	685
				
	ggr-2	GGR_F2	CGGATTTCTATGGTTGGGTG	
		SAIL_LB2	GCTTCCTATTATATCTTCCCAAATTACCAATACA	876
				
	WRI1	WRI1_F1	CATCACCACAACGGAAGATG	
	(in wri1-4 mutant)	WRI1_R1	AGATGAGCAGTGCACGTGAG	1091
				
	WRI1	WRI1_F2	ACTGCTTTCACGCGTAATTG	
	(in wri1-3 mutant)	WRI1_R2	GCCTCACGTGAGTCAGTGTG	1017
				
	wri1-3	WRI1_F2	ACTGCTTTCACGCGTAATTG	
		LBb1.3	ATTTTGCCGATTTCGGAAC	626
				
	wri1-4	WRI1_R1	AGATGAGCAGTGCACGTGAG	
		LBb1.3	ATTTTGCCGATTTCGGAAC	661
				
	DGAT1	DGAT1_F	TTTTGACAGCTATGCATCGC	
		DGAT1_R	AGTGTTTGCTGCACATCGAC	1023
				
	dgat1-4	DGAT1_F	TTTTGACAGCTATGCATCGC	
		LBb1.3	ATTTTGCCGATTTCGGAAC	622
				
	VTE5	VTE5_F	TTCAGACCATGTTGAAGAATGG 	
		VTE5_R	ACCACTTTGTTCTTGTCCGC	1172
				
	vte5-3	VTE5_F	TTCAGACCATGTTGAAGAATGG	
		LBb1.3	ATTTTGCCGATTTCGGAAC	746
				
	vte5-4	VTE5_F	TTCAGACCATGTTGAAGAATGG	
		L4	CGTGAAGTTTCTCATCTAAGCCCC	500
				
	eve1	EVE1_F	CTCTAAATATCGCGGCGTCG	348 (WT)
		EVE1_R	CTTCCGATCCGAGCCTCCCATCTTCCGTTGTGGTGAAGC	310 + 38 (eve1)
				
	eve4	EVE4_R	TCTCTGCTTCGGGGATCGTG	324 (WT)
		EVE4_F	CTTGCTGCGCAAGCACGGGAAGTATATATGTCGAACGAT	286 + 38 (eve4)
				
				
	Allele indicates the genotype of DNA template amplified by PCR with these two primers			
				
	Band size indicate the size of the PCR products otained with these two primers