Zimmermann, Petra Stefano, Leo Faruk Cetiner, Omer Pittet, Laure F. Messina, Nicole L. Jakob, William Falquet, Laurent Curtis, Nigel 2023-03-16 Introduction: The early-life intestinal microbiome plays a crucial role in the development and regulation of the immune system. Perturbations in its composition during this critical period have been linked to the development of allergic diseases. Objective: This study aimed to investigate the association between the composition of the early-life intestinal microbiome and the presence of eczema in the first year of life using shotgun metagenomic sequencing and functional analyses (metabolic pathways). Methods: Stool samples from 393 healthy term infants collected at 1 week of age were analyzed with shotgun metagenomic sequencing. Environmental and clinical data were prospectively collected using 3-monthly validated questionnaires. Participants were clinically assessed during study visits at 12 months of age. Eczema was diagnosed by the UK diagnostic tool and by a research nurse. Data analysis was stratified by delivery mode. Results: Eczema was diagnosed in 16.4% (60/366) of participants by nurse diagnosis. Infants born by cesarean section (CS) with nurse-diagnosed eczema had a higher relative abundance of Escherichia, Shigella, Enterobacter, and Citrobacter and a lower relative abundance of Veillonella than CS-born infants without eczema. In addition, CS-born infants without eczema had a higher abundance of genes involved in lactic fermentation. Vaginally born infants with eczema had a higher relative abundance of Bacteroides and a lower abundance of Streptococcus. Conclusion: There is an association between the bacterial composition of the intestinal microbiome at 1 week of age and the presence of eczema in the first 12 months of life. application/pdf https://folia.unifr.ch/global/documents/325438 https://folia.unifr.ch/documents/325438/files/leo_microbiomeandeczema_frontmicrob_2023.pdf https://folia.unifr.ch/documents/325438/files/DataSheet_3_The association between the composition of the early-life intestinal microbiome and eczema in the first year of life.xlsx https://folia.unifr.ch/documents/325438/files/DataSheet_2_The association between the composition of the early-life intestinal microbiome and eczema in the first year of life.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.3389/frmbi.2023.1147082 info:eu-repo/semantics/altIdentifier/issn/2813-4338 info:eu-repo/semantics/openAccess CC BY Frontiers in Microbiomes. - Frontiers Media SA. - 2023, vol. 2 atopic dermatitis allergies microbiota stool intestine shotgun metagenomic sequencing infants gut info:eu-repo/classification/udc/616 The association between the composition of the early-life intestinal microbiome and eczema in the first year of life http://purl.org/coar/resource_type/c_6501