Aires-de-Sousa, Marta Fournier, Claudine Lopes, Elizeth Lencastre, Hermínia de Nordmann, Patrice Poirel, Laurent 2020-09-28 In order to evaluate whether seagulls living on the Lisbon coastline, Portugal, might be colonized and consequently represent potential spreaders of multidrug-resistant bacteria, a total of 88 gull fecal samples were screened for detection of extended- spectrum β-lactamase (ESBL)- or carbapenemase-producing Enterobacteriaceae for methicillin-resistant Staphylococcus aureus (MRSA) and for vancomycin-resistant Enterococci (VRE). A large proportion of samples yielded carbapenemase- or ESBL- producing Enterobacteriaceae (16% and 55%, respectively), while only two MRSA and two VRE were detected. Mating-out assays followed by PCR and whole-plasmid sequencing allowed to identify carbapenemase and ESBL encoding genes. Among 24 carbapenemase-producing isolates, there were mainly Klebsiella pneumoniae (50%) and Escherichia coli (33%). OXA-181 was the most common carbapenemase identified (54%), followed by OXA-48 (25%) and KPC-2 (17%). Ten different ESBLs were found among 62 ESBL-producing isolates, mainly being CTX-M-type enzymes (87%). Co-occurrence in single samples of multiple ESBL- and carbapenemase producers belonging to different bacterial species was observed in some cases. Seagulls constitute an important source for spreading multidrug-resistant bacteria in the environment and their gut microbiota a formidable microenvironment for transfer of resistance genes within bacterial species. application/pdf https://folia.unifr.ch/global/documents/309047 https://folia.unifr.ch/documents/309047/files/poi_hcr.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms8101487 info:eu-repo/semantics/openAccess License undefined Microorganisms. - 2020, vol. 8, no. 10, p. 1487 info:eu-repo/classification/udc/57 High colonization rate and heterogeneity of ESBL- and carbapenemase-producing enterobacteriaceae isolated from gull feces in Lisbon, Portugal http://purl.org/coar/resource_type/c_6501