Sadek, Mustafa Demord, Anthony Poirel, Laurent Nordmann, Patrice 2020-11-01 The Revogene® Carba C assay is a real-time polymerase chain reaction–based assay that runs on the microfluidic Revogene platform. It was recently designed for the detection of genes encoding the 5 major carbapenemases (NDM, VIM, IMP, KPC, and OXA-48) from various Gram-negatives. A total of 145 clinical Gram-negative strains (96 carbapenemase producers and 49 non–carbapenemase producers) were tested. The overall sensitivity and specificity were 100%. All strains co-producing double carbapenemases have been correctly detected. All non–carbapenemase producers and nontargeted carbapenemase producers gave a negative result. The sample preparation was easy to handle, taking around 5 to 10 min per isolate, with a run time of approximately 70 min. This assay is a rapid, easy-to-perform, reliable tool to detect the most common carbapenemases, with excellent sensitivity and specificity regardless of the host bacteria. Given its user friendliness, simplicity, and short time to result, the Revogene® Carba C assay is suitable for microbiology laboratories. application/pdf https://folia.unifr.ch/global/documents/309041 https://folia.unifr.ch/documents/309041/files/nor_frd.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.diagmicrobio.2020.115127 info:eu-repo/semantics/openAccess License undefined Diagnostic Microbiology and Infectious Disease. - 2020, vol. 98, no. 3, p. 115127 info:eu-repo/classification/udc/57 Fast and reliable detection of carbapenemase genes in various Gram negatives using a new commercially available fluorescence-based real-time polymerase chain reaction platform http://purl.org/coar/resource_type/c_6501