Sadek, Mustafa Nariya, Hirofumi Shimamoto, Toshi Kayama, Shizuo Yu, Liansheng Hisatsune, Junzo Sugai, Motoyuki Nordmann, Patrice Poirel, Laurent Shimamoto, Tadashi 2020-08-22 We describe here the complete genome sequence of an Enterobacter hormaechei ST279 coharbouring blaVIM-1 and mcr-9 recovered from uncooked beef patty in June 2017, Egypt. The tested isolate was resistant to carbapenem but susceptible to colistin (minimum inhibitory concentration (MIC), 0.5 μg/mL). The antimicrobial susceptibility profile and conjugation experiments were performed. The entire genome was sequenced by the Illumina MiniSeq and Oxford Nanopore methods. The blaVIM-1 and mcr-9 genes are carried on the same IncHI2/pMLST1 plasmid, pMS37a (Size of 270.9 kb). The mcr-9 gene was located within the physical boundaries demarcated by two insertion elements IS903 (upstream) and IS1 (downstream) but did not possess the downstream regulatory genes (qseC/qseB) which regulate the expression of mcr- 9. Therefore, the mcr-9 might be silently disseminated among carbapenem-resistant Enterobacterales. In addition to blaVIM-1 and mcr-9, plasmid pMS37a harbored various antibiotic resistance genes including aac(6’)-Il, ΔaadA22, aac(6’)-Ib-cr, sul1, dfrA1 and tetA. To the best of our knowledge, this is the first report of a blaVIM-1 and mcr-9-coharbouring E. hormaechei isolate of food origin worldwide. The identification of a multidrug-resistant VIM-1 and mcr-9 positive Enterobacter hormaechei isolate from food is worrisome as retail meat and meat products could serve as a vehicle for these MDR bacteria, which could be transferred between animals and humans through the food chain. It further highlights that Enterobacterales co-producing MCR and carbapenemases being found in the food chain indeed correspond to a One- Health issue, highlighting the need for serious steps to prevent their further dissemination. application/pdf https://folia.unifr.ch/global/documents/309034 https://folia.unifr.ch/documents/309034/files/nor_fgc.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.3390/pathogens9090687 info:eu-repo/semantics/openAccess License undefined Pathogens. - 2020, vol. 9, no. 9, p. 687 info:eu-repo/classification/udc/57 First genomic characterization of blavim-1 and mcr-9-coharbouring enterobacter hormaechei isolated from food of animal origin http://purl.org/coar/resource_type/c_6501