Fournier, Claudine Poirel, Laurent Nordmann, Patrice 2020-10-01 Carbapenem-resistant Acinetobacter baumannii (CRAB) and Pseudomonas aeruginosa (CRPA), as well as polymyxin-resistant A. baumannii (PMR-AB) and P. aeruginosa (PMR-PA), were used to test different enrichment strategies from spiked stools. Three procedures were compared, namely, direct inoculation on selective plates and plating after a 24-h enrichment step in tryptic soy broth with or without antibiotics. Selective agar plates were used including CHROMagar-Pseudomonas supplemented with meropenem (2 mg/L), and CHROMagar-MDR-Acinetobacter agar and CHROMagar COL-APSE plates. Use of enrichment broths significantly enhanced the recovery of CRAB, CRPA, PMR-AB, and PMR-PA. However, supplementing or not the pre-enrichment broth with antibiotics had no impact. The proposed strategy for screening multidrug-resistant nonfermenters is of low cost, is easy to implement, and might be useful for outbreak containment. application/pdf https://folia.unifr.ch/global/documents/308992 https://folia.unifr.ch/documents/308992/files/nor_iem.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.diagmicrobio.2020.115121 info:eu-repo/semantics/openAccess License undefined Diagnostic Microbiology and Infectious Disease. - 2020, vol. 98, no. 2, p. 115121 info:eu-repo/classification/udc/57 Implementation and evaluation of methods for the optimal detection of carbapenem-resistant and colistin-resistant Pseudomonas aeruginosa and Acinetobacter baumannii from stools http://purl.org/coar/resource_type/c_6501