<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Piza-Buitrago, Adriana</dc:creator>
  <dc:creator>Rincón, Verónica</dc:creator>
  <dc:creator>Donato, John</dc:creator>
  <dc:creator>Saavedra, Sandra Yamile</dc:creator>
  <dc:creator>Duarte, Carolina</dc:creator>
  <dc:creator>Morero, Jaime</dc:creator>
  <dc:creator>Falquet, Laurent</dc:creator>
  <dc:creator>Reguero, María Teresa</dc:creator>
  <dc:creator>Barreto-Hernández, Emiliano</dc:creator>
  <dc:date>2020-11-12</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">Providencia rettgeri is a nosocomial pathogen associated with urinary tract infections  and related to Healthcare-Associated Infection (HAI). In recent years isolates  producing New Delhi Metallo-β-lactamase (NDM) and other β-lactamases  have been reported that reduce the efficiency of clinical antimicrobial treatments. In  this study, we analyzed antibiotic resistance, the presence of resistance genes and  the clonal relationship of two P. rettgeri isolates obtained from male  patients admitted to the same hospital in Bogotá – Colombia, 2015. Results: Antibiotic susceptibility profile evaluated by the Kirby-Bauer method revealed  that both isolates were resistant to third-generation carbapenems and cephalosporins. Whole-genome sequencing (Illumina HiSeq) followed by SPAdes assembling, Prokka  annotation in combination with an in-house Python program and resistance gene  detection by ResFinder identified the same six β-lactamase genes in  both isolates: blaNDM-1, blaVIM-2, blaCTX-M-15, blaOXA-10, blaCMY-2 and blaTEM- 1. Additionally, various resistance genes associated with antibiotic target alteration  (arnA, PmrE, PmrF, LpxA, LpxC, gyrB, folP, murA, rpoB, rpsL, tet34)  were found and four efflux pumps (RosAB, EmrD, mdtH and cmlA).  The additional resistance to gentamicin in one of the two isolates could be explained  by a detected SNP in CpxA (Cys191Arg) which is involved in the stress response of  the bacterial envelope. Genome BLAST comparison using CGView, the ANI value (99.99%) and the  pangenome (using Roary) phylogenetic tree (same clade, small distance) showed  high similarity between the isolates. The rMLST analysis indicated that both  isolates were typed as rST-61,696, same as the RB151 isolate previously isolated in  Bucaramanga, Colombia, 2013, and the FDAARGOS_330 isolate isolated in the USA,  2015. Conclusions: We report the coexistence of the carbapenemase genes blaNDM-1, and  blaVIM-2, together with the β-lactamase genes blaCTX-M-15, blaOXA-10, blaCMY-2  and blaTEM-1, in P. rettgeri isolates from two patients in  Colombia. Whole-genome sequence analysis indicated a circulation of P. rettgeri rST- 61,696 strains in America that needs to be investigated further.</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>https://folia.unifr.ch/global/documents/308964</dc:identifier>
  <dc:identifier>https://folia.unifr.ch/documents/308964/files/bmc_20.pdf</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1186/s12866-020-02030-z</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>License undefined</dc:rights>
  <dc:source>BMC Microbiology. - 2020, vol. 20, no. 1, p. 345</dc:source>
  <dc:subject>info:eu-repo/classification/udc/57</dc:subject>
  <dc:title xmlns:ns1="xml" ns1:lang="en">Genome-based characterization of two Colombian clinical Providencia rettgeri isolates co-harboring NDM-1, VIM-2, and other β-lactamases</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>