Zhou, Ying Tang, Yu Fu, Pan Tian, Dongxing Yu, Lianhua Huang, Yunkun Li, Gang Li, Meng Wang, Yong Yang, Zehua Xu, Xiaogang Yin, Zhe Zhou, Dongsheng Poirel, Laurent Jiang, Xiaofei 2020-05-20 Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and bla KPC-IncF plasmids. CRISPR-Cas system is an adaptive immune system that can hinder gene expansion driven by horizontal gene transfer. Because of bla KPC-IncF plasmids are favored by CG258 K. pneumoniae, it was of interest to examine the co-distribution of CRISPR and bla KPC-IncF plasmids in such isolates. We collected 459 clinical K. pneumoniae isolates in China and collected 203 global whole-genome sequences in GenBank to determine the prevalence of CRISPR-Cas systems. We observed that CRISPR-Cas system was significantly scarce in the CG258 lineage and bla KPC-positive isolates. Furthermore, the results of conjugation and plasmid stability assay fully demonstrated the CRIPSR- Cas system in K. pneumoniae could effectively hindered bla KPC-IncF plasmids invasion and existence. Notably, most bla KPC-IncF plasmids were also proved to be good targets of CRISPR owing to carry matched and functional protospacers and PAMs. Overall, our work suggests that type I-E CRISPR-Cas systems could impact the spread of bla KPC in K. pneumoniae populations, and the scarcity of CRISPR-Cas system was one of potential factors leading to the propagation of bla KPC-IncF plasmids in CG258 K. pneumoniae. application/pdf https://folia.unifr.ch/global/documents/308807 https://folia.unifr.ch/documents/308807/files/poi_tic.pdf https://folia.unifr.ch/documents/308807/files/poi_tic_sm.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1080/22221751.2020.1763209 info:eu-repo/semantics/openAccess License undefined Emerging Microbes & Infections. - 2020, vol. 9, no. 1, p. 1011–1022 info:eu-repo/classification/udc/57 The type I-E CRISPR-Cas system influences the acquisition of bla KPC-IncF plasmid in Klebsiella pneumonia http://purl.org/coar/resource_type/c_6501