Masseron, Amandine Poirel, Laurent Falgenhauer, Linda Imirzalioglu, Can Kessler, Julie Chakraborty, Trinad Nordmann, Patrice 2020-07-01 OXA-244 is a derivative of OXA-48 showing weaker carbapenemase activity, compromising the detection of corresponding producers in clinical laboratories. Since 2017, the Swiss National Reference Center for Emerging Antibiotic Resistance noticed an increased identification of OXA-244-producing Escherichia coli (n=15) within the country. Different methods (biochemical and immunoassay tests, screening culture media) were tested for the detection of OXA-244 producers. Whole genome sequencing was used to investigate the genetic relatedness between the isolates and the genetic structures at the origin of the acquisition of the blaOXA-244 gene. The mSuperCARBA® medium and the NG-Test CARBA5 assay were found to be suitable tools for detecting all OXA-244-producing isolates. Other selective media did not perform optimally. Among the fifteen strains, five sequence types were identified, with ST38 being predominant. The blaOXA-244 gene was located on the chromosome for all isolates. Overall, detection of OXA-244 producers is challenging and specific guidelines must be followed. application/pdf https://folia.unifr.ch/global/documents/308713 https://folia.unifr.ch/documents/308713/files/nor_odo.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.diagmicrobio.2020.115059 info:eu-repo/semantics/openAccess License undefined Diagnostic Microbiology and Infectious Disease. - 2020, vol. 97, no. 3, p. 115059 info:eu-repo/classification/udc/57 Ongoing dissemination of OXA-244 carbapenemase-producing Escherichia coli in Switzerland and their detection http://purl.org/coar/resource_type/c_6501