Magalhães, Joana Pinheiro, Marina Drasler, Barbara Septiadi, Dedy Petri-Fink, Alke Santos, Susana G Rothen-Rutishauser, Barbara Reis, Salette 2020-02-01 Aim: Design nanostructured lipid carriers (NLC) to facilitate drug delivery to tuberculosis-infected areas, exploiting macrophage mannose receptors and assess their uptake in a 3D human lung model. Materials & methods: NLCs and mannosylated-NLCs were synthetized and characterized. Their uptake and biocompatibility were tested in a 3D human lung model. Results: The formulations have appropriate size (170–202 nm) and morphology for lung deposition. Cell membrane integrity was maintained and no significant pro- inflammatory cytokine (IL-1β, IL-8 and TNF-α) secretion or morphological changes were observed 24 h post nanoparticles exposure. NLCs and mannosylated NLCs were distributed in the apical side of the lung tissue, both in macrophages and in epithelial cells. Conclusion: NLCs are biocompatible carriers and can be used for pulmonary drug delivery. application/pdf https://folia.unifr.ch/global/documents/308615 https://folia.unifr.ch/documents/308615/files/fin_lnb.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.2217/nnm-2019-0256 info:eu-repo/semantics/openAccess License undefined Nanomedicine. - 2020, vol. 15, no. 3, p. 259–271 info:eu-repo/classification/udc/54 Lipid nanoparticles biocompatibility and cellular uptake in a 3D human lung model http://purl.org/coar/resource_type/c_6501