Sadek, Mustafa Tinguely, Camille Poirel, Laurent Nordmann, Patrice 2020-04-21 Polymyxins are becoming an added value for treating infections due to multidrug-resistant Pseudomonas aeruginosa. Therefore, the Rapid Polymyxin/Pseudomonas NP test was developed for the identification of polymyxin resistance in P. aeruginosa isolates. This test detects the bacterial growth in a medium containing a defined concentration of colistin. Formation of basic metabolites is visually detected by yellow to purple/violet color change of the bromocresol purple pH indicator. The test performance was evaluated by using 50 colistin-resistant and colistin-susceptible P. aeruginosa isolates, among which 10 were colistin resistant. The sensitivity and specificity of the test were found to be 100% (CI95 65.5-100%) and 95% (CI95 81.8-99.1%), respectively, by comparison with broth microdilution as the reference method. The Rapid Polymyxin/Pseudomonas NP test is easy to perform, specific and sensitive, allowing rapid visual observation of results without the requirement any special reading equipment, and results are obtained in 3 h. application/pdf https://folia.unifr.ch/global/documents/308537 https://folia.unifr.ch/documents/308537/files/nor_rpp.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1007/s10096-020-03884-x info:eu-repo/semantics/openAccess License undefined European Journal of Clinical Microbiology & Infectious Diseases. - 2020, vol. 39, no. 9, p. 1657-1662 info:eu-repo/classification/udc/57 Rapid polymyxin/pseudomonas NP test for rapid detection of polymyxin susceptibility/resistance in Pseudomonas aeruginosa http://purl.org/coar/resource_type/c_6501