Poirel, Laurent Vuillemin, Xavier Kieffer, Nicolas Mueller, Linda Descombes, Marie-Christine Nordmann, Patrice 2019-11-01 A plasmid-located fosfomycin resistance gene, fosA8, was identified from a CTX-M- 15-producing Escherichia coli isolate recovered from urine. Identification of this gene was obtained by whole-genome sequencing. It encoded FosA8, which shares 79% and 78% amino acid identity with the most closely related FosA2 and FosA1 enzymes, respectively. The fosA8 gene was located on a transferable 50-kb plasmid of IncN type encoding high-level resistance to fosfomycin. In silico analysis and cloning experiments identified fosA8 analogues (99% identity) in the genome of Leclercia decarboxylata, which is an enterobacterial species with natural resistance to fosfomycin. This finding adds L. decarboxylata to the list of enterobacterial species that are a reservoir of fosA-like genes which have been captured from the chromosome of a progenitor and are then acquired by E. coli. application/pdf https://folia.unifr.ch/global/documents/308400 https://folia.unifr.ch/documents/308400/files/nor_ifp.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1128/AAC.01403-19 info:eu-repo/semantics/openAccess License undefined Antimicrobial Agents and Chemotherapy. - 2019, vol. 63, no. 11, p. 10.1128/aac.01403-19 info:eu-repo/classification/udc/57 Identification of fosa8, a plasmid-encoded fosfomycin resistance determinant from Escherichia coli, and its origin in Leclercia adecarboxylata http://purl.org/coar/resource_type/c_6501