Bontron, Séverine Poirel, Laurent Kieffer, Nicolas Savov, Encho Trifonova, Angelina Todorova, Iva Kueffer, Gwendoline Nordmann, Patrice 2019-05-30 The aim of the study was to decipher the mechanisms and associated genetic determinants responsible for increased carbapenem resistance among Proteus mirabilis clinical isolates.Methods: The entire genetic structure surrounding the β- lactam resistance genes was characterized by PCR, gene walking, and DNA sequencing.Results: A series of clinical P. mirabilis isolates were consecutively recovered from different patients at the Military hospital of Sofia, Bulgaria. They showed variable levels of resistance to carbapenems. All isolates produced the same carbapenemase VIM-1 that was chromosomally encoded. We showed that increased resistance to carbapenems was related to an increased number of blaVIM-1 gene copies.Conclusion: We showed here that increased carbapenem resistance in P. mirabilis may result from increased expression of the blaVIM-1 carbapenemase gene through multiplication of its copy number application/pdf https://folia.unifr.ch/global/documents/308376 https://folia.unifr.ch/documents/308376/files/nor_irc.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1089/mdr.2018.0365 info:eu-repo/semantics/openAccess License undefined Microbial Drug Resistance. - 2019, vol. 25, no. 5, p. 663–667 info:eu-repo/classification/udc/57 Increased resistance to carbapenems in proteus mirabilis mediated by amplification of the bla vim-1 -carrying and is 26 -associated class 1 integron http://purl.org/coar/resource_type/c_6501