Girardin, Ariane Wang, Tongming Ding, Yi Keller, Jean Buendia, Luis Gaston, Mégane Ribeyre, Camille Gasciolli, Virginie Auriac, Marie-Christine Vernié, Tatiana Bendahmane, Abdelhafid Ried, Martina Katharina Parniske, Martin Morel, Patrice Vandenbussche, Michiel Schorderet, Martine Reinhardt, Didier Delaux, Pierre-Marc Bono, Jean-Jacques Lefebvre, Benoit 2019-12-16 Bacterial lipo-chitooligosaccharides (LCOs) are key mediators of the nitrogen-fixing root nodule symbiosis (RNS) in legumes. The isolation of LCOs from arbuscular mycorrhizal fungi suggested that LCOs are also signaling molecules in arbuscular mycorrhiza (AM). However, the corresponding plant receptors have remained uncharacterized. Here we show that petunia and tomato mutants in the LysM receptor-like kinases LYK10 are impaired in AM formation. Petunia and tomato LYK10 proteins have a high affinity for LCOs (Kd in the nM range) comparable to that previously reported for a legume LCO receptor essential for the RNS. Interestingly, the tomato and petunia LYK10 promoters, when introduced into a legume, were active in nodules similarly to the promoter of the legume orthologous gene. Moreover, tomato and petunia LYK10 coding sequences restored nodulation in legumes mutated in their orthologs. This combination of genetic and biochemical data clearly pinpoints Solanaceous LYK10 as part of an ancestral LCO perception system involved in AM establishment, which has been directly recruited during evolution of the RNS in legumes. application/pdf https://folia.unifr.ch/global/documents/308340 https://folia.unifr.ch/documents/308340/files/rei_lri.pdf https://folia.unifr.ch/documents/308340/files/rei_lri_sm.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cub.2019.11.038 info:eu-repo/semantics/openAccess License undefined Current Biology. - 2019, vol. 29, no. 24, p. 4249-4259.e5 info:eu-repo/classification/udc/57 LCO receptors involved in arbuscular mycorrhiza are functional for rhizobia perception in legumes http://purl.org/coar/resource_type/c_6501