Brunet Avalos, Clarisse Maier, G. Larisa Bruggmann, Rémy Sprecher, Simon G. 2019-11-20 Cell diversity of the brain and how it is affected by starvation, remains largely unknown. Here, we introduce a single cell transcriptome atlas of the entire Drosophila first instar larval brain. We first assigned cell-type identity based on known marker genes, distinguishing five major groups: neural progenitors, differentiated neurons, glia, undifferentiated neurons and non-neural cells. All major classes were further subdivided into multiple subtypes, revealing biological features of various cell-types. We further assessed transcriptional changes in response to starvation at the single- cell level. While after starvation the composition of the brain remains unaffected, transcriptional profile of several cell clusters changed. Intriguingly, different cell-types show very distinct responses to starvation, suggesting the presence of cell-specific programs for nutrition availability. Establishing a single-cell transcriptome atlas of the larval brain provides a powerful tool to explore cell diversity and assess genetic profiles from developmental, functional and behavioral perspectives. application/pdf https://folia.unifr.ch/global/documents/308181 https://folia.unifr.ch/documents/308181/files/spr_sct.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.50354 info:eu-repo/semantics/openAccess License undefined eLife. - 2019, vol. 8, p. e50354 info:eu-repo/classification/udc/57 Single cell transcriptome atlas of the Drosophila larval brain http://purl.org/coar/resource_type/c_6501