Mitterer, Valentin Shayan, Ramtin Ferreira-Cerca, Sébastien Murat, Guillaume Enne, Tanja Rinaldi, Dana Weigl, Sarah Omanic, Hajrija Gleizes, Pierre-Emmanuel Kressler, Dieter Plisson-Chastang, Celia Pertschy, Brigitte 2019 Eukaryotic ribosomes are synthesized in a hierarchical process driven by a plethora of assembly factors, but how maturation events at physically distant sites on pre- ribosomes are coordinated is poorly understood. Using functional analyses and cryo- EM, we show that ribosomal protein Rps20 orchestrates communication between two multi-step maturation events across the pre-40S subunit. Our study reveals that during pre-40S maturation, formation of essential contacts between Rps20 and Rps3 permits assembly factor Ltv1 to recruit the Hrr25 kinase, thereby promoting Ltv1 phosphorylation. In parallel, a deeply buried Rps20 loop reaches to the opposite pre- 40S side, where it stimulates Rio2 ATPase activity. Both cascades converge to the final maturation steps releasing Rio2 and phosphorylated Ltv1. We propose that conformational proofreading exerted via Rps20 constitutes a checkpoint permitting assembly factor release and progression of pre-40S maturation only after completion of all earlier maturation steps. application/pdf https://folia.unifr.ch/global/documents/307989 https://folia.unifr.ch/documents/307989/files/kre_cpd.pdf https://folia.unifr.ch/documents/307989/files/kre_cpd_sm.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-019-10678-z info:eu-repo/semantics/openAccess License undefined Nature Communications. - 2019, vol. 10, no. 1, p. 2754 info:eu-repo/classification/udc/57 Conformational proofreading of distant 40S ribosomal subunit maturation events by a long-range communication mechanism http://purl.org/coar/resource_type/c_6501