Sønder, Stine Lauritzen Boye, Theresa Louise Tölle, Regine Dengjel, Jörn Maeda, Kenji Jäättelä, Marja Simonsen, Adam Cohen Jaiswal, Jyoti K. Nylandsted, Jesper 2019-04-30 The plasma membrane of eukaryotic cells forms the essential barrier to the extracellular environment, and thus plasma membrane disruptions pose a fatal threat to cells. Here, using invasive breast cancer cells we show that the Ca2+ - and phospholipid-binding protein annexin A7 is part of the plasma membrane repair response by enabling assembly of the endosomal sorting complex required for transport (ESCRT) III. Following injury to the plasma membrane and Ca2+ flux into the cytoplasm, annexin A7 forms a complex with apoptosis linked gene-2 (ALG-2) to facilitate proper recruitment and binding of ALG-2 and ALG-2-interacting protein X (ALIX) to the damaged membrane. ALG-2 and ALIX assemble the ESCRT III complex, which helps excise and shed the damaged portion of the plasma membrane during wound healing. Our results reveal a novel function of annexin A7 – enabling plasma membrane repair by regulating ESCRT III-mediated shedding of injured plasma membrane. application/pdf https://folia.unifr.ch/global/documents/307815 https://folia.unifr.ch/documents/307815/files/den_are.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-019-43143-4 info:eu-repo/semantics/openAccess License undefined Scientific Reports. - 2019, vol. 9, no. 1, p. 6726 info:eu-repo/classification/udc/57 Annexin A7 is required for ESCRT III-mediated plasma membrane repair http://purl.org/coar/resource_type/c_6501