Lescat, Mathilde Poirel, Laurent Tinguely, Camille Nordmann, Patrice 2018-12-19 A rapid test was developed for identification of polymyxin resistance in non-fermenting bacteria. This test detects viable cells after growth in a medium containing a defined concentration of colistin. The principle of this test is based on the visual detection of the reduction of the resazurin reagent, a viability colorant, observed by its color change (blue to purple or pink). Its evaluation was performed by using 92 colistin- resistant and colistin-susceptible Acinetobacter baumannii and Pseudomonas aeruginosa isolates. Sensitivity and specificity were respectively found to be 100 and 95% by comparison with the standard broth microdilution method. The Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test is inexpensive, easy-to-perform, highly sensitive and specific, and can be completed in 4 hours. It could be useful in countries facing endemic spread of colistin-resistant non fermenters. application/pdf https://folia.unifr.ch/global/documents/307791 https://folia.unifr.ch/documents/307791/files/nor_rrb.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1128/JCM.01563-18 info:eu-repo/semantics/openAccess License undefined Journal of Clinical Microbiology. - 2019, vol. 57, no. 3, p. 10.1128/jcm.01563-18 info:eu-repo/classification/udc/57 A resazurin reduction-based assay for rapid detection of polymyxin resistance in Acinetobacter baumannii and Pseudomonas aeruginosa http://purl.org/coar/resource_type/c_6501