Mueller, Linda Cimen, Cansu Poirel, Laurent  Descombes, Marie-Christine Nordmann, Patrice 2019-05-01 Our aim was to evaluate the prevalence of fosfomycin-resistant strains among ESBL- producing Escherichia coli isolates recovered from community patients in Switzerland. A total of 1225 ESBL-producing E. coli isolates were collected between 2012 and 2013 from a private and community laboratory. Fosfomycin resistance was assessed by using the novel rapid fosfomycin/E. coli NP test and agar dilution method. Resistant isolates were further investigated for acquired resistance genes fosA1–7 by PCR and sequencing. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were performed to evaluate the clonal relationship among fosA3-carrying isolates. Out of the 1225 ESBL-producing E. coli isolates analyzed in this study, 1208 were fosfomycin susceptible while 17 were fosfomycin resistant. No discrepancy was observed between the rapid fosfomycin/E. coli NP test and the agar dilution method taken as the gold standard. Five out of the 17 resistant isolates carried a fosA-like gene. No clonal relationship was observed among those isolates. Here, the prevalence of fosfomycin resistance among ESBL-producing E. coli isolates in the community is reported for the first time in Switzerland, being ca. 1.4%. Among the five isolates carrying a fosA gene, four encoded the FosA3 enzyme, being the most prevalent fosfomycin-resistant determinant. An excellent correlation was observed between minimum inhibitory concentration–based susceptibility categorization and results of the rapid fosfomycin/E. coli NP test, further indicating the excellent sensitivity and specificity of this recently developed rapid test whose results are obtained in less than 2 h. application/pdf https://folia.unifr.ch/global/documents/307768 https://folia.unifr.ch/documents/307768/files/nor_pfr.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1007/s10096-019-03531-0 info:eu-repo/semantics/openAccess License undefined European Journal of Clinical Microbiology & Infectious Diseases. - 2019, vol. 38, no. 5, p. 945–949 info:eu-repo/classification/udc/57 Prevalence of fosfomycin resistance among ESBL-producing Escherichia coli isolates in the community, Switzerland http://purl.org/coar/resource_type/c_6501