Harpe, Marylaure de La Hess, Jaqueline Loiseau, Oriane Salamin, Nicolas Lexer, Christian Paris, Margot 2019 Understanding the genetics of biological diversification across micro‐ and macro‐ evolutionary time scales is a vibrant field of research for molecular ecologists as rapid advances in sequencing technologies promise to overcome former limitations. In palms, an emblematic, economically and ecologically important plant family with high diversity in the tropics, studies of diversification at the population and species levels are still hampered by a lack of genomic markers suitable for the genotyping of large numbers of recently diverged taxa. To fill this gap, we used a whole genome sequencing approach to develop target sequencing for molecular markers in 4,184 genome regions, including 4,051 genes and 133 non‐genic putatively neutral regions. These markers were chosen to cover a wide range of evolutionary rates allowing future studies at the family, genus, species and population levels. Special emphasis was given to the avoidance of copy number variation during marker selection. In addition, a set of 149 well‐known sequence regions previously used as phylogenetic markers by the palm biological research community were included in the target regions, to open the possibility to combine and jointly analyse already available data sets with genomic data to be produced with this new toolkit. The bait set was effective for species belonging to all three palm sub‐families tested (Arecoideae, Ceroxyloideae and Coryphoideae), with high mapping rates, specificity and efficiency. The number of high‐quality single nucleotide polymorphisms (SNPs) detected at both the sub‐family and population levels facilitates efficient analyses of genomic diversity across micro‐ and macro‐evolutionary time scales. application/pdf https://folia.unifr.ch/global/documents/307565 https://folia.unifr.ch/documents/307565/files/par_dtc.pdf https://folia.unifr.ch/documents/307565/files/par_dtc_sm1.txt https://folia.unifr.ch/documents/307565/files/par_dtc_sm2.txt https://folia.unifr.ch/documents/307565/files/par_dtc_sm3.txt eng info:eu-repo/semantics/altIdentifier/doi/10.1111/1755-0998.12945 info:eu-repo/semantics/openAccess License undefined Molecular Ecology Resources. - 2019, vol. 19, no. 1, p. 221-234 info:eu-repo/classification/udc/57 A dedicated target capture approach reveals variable genetic markers across micro- and macro-evolutionary time scales in palms http://purl.org/coar/resource_type/c_6501