Poirel, Laurent Aires-de-Sousa, Marta Kudyba, Patrick Kieffer, Nicolas Nordmann, Patrice 2018-09-01 The occurrence of resistance to last-resort antibiotics was evaluated among Enterobacteriaceae isolates recovered from hospitalized children in a remote African archipelago, São Tomé and Príncipe, where there is limited access to those antibiotics. Fifty patients were screened for colonization by carbapenem-, pan- aminoglycoside-, or polymyxin-resistant Enterobacteriaceae. A total of 36 isolates (including 30 Escherichia coli and 4 Klebsiella pneumoniae) were recovered from 23 patients, including 26 isolates harboring the blaOXA-181 carbapenemase gene, a single isolate harboring the 16S rRNA methylase gene rmtB encoding pan-resistance to aminoglycosides, and 8 isolates coharboring both genes. A single isolate possessed the plasmid-borne colistin resistance gene mcr-1. A high clonal relationship was found for OXA-181-producing E. coli (4 clones), and conversely, three of the four OXA-181-producing K. pneumoniae isolates were clonally unrelated. This study overall showed a high prevalence of resistance to last-resort antibiotics in this country, where no epidemiological data were previously available. application/pdf https://folia.unifr.ch/global/documents/307455 https://folia.unifr.ch/documents/307455/files/nor_scm.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1128/AAC.01021-18 info:eu-repo/semantics/openAccess License undefined Antimicrobial Agents and Chemotherapy. - 2018, vol. 62, no. 9, p. e01021-18 info:eu-repo/classification/udc/57 Screening and characterization of multidrug-resistant gram-negative bacteria from a remote african area, São Tomé and Príncipe http://purl.org/coar/resource_type/c_6501