<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Blanchoud, Simon</dc:creator>
  <dc:creator>Zondag, Lisa</dc:creator>
  <dc:creator>Lamare, Miles D.</dc:creator>
  <dc:creator>Wilson, Megan J.</dc:creator>
  <dc:date>2017-06-01</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">Whole-body regeneration (WBR)—the formation of an entire adult from only a small  fragment of its own tissue—is extremely rare among chordates. Exceptionally, in the  colonial ascidian Botrylloides leachii (Savigny, 1816) a fully functional adult is formed  from their common vascular system after ablation of all adults from the colony in just  10 d, thanks to their high blastogenetic potential. While previous studies have  identified key genetic markers and morphological changes, no study has yet focused  on the hematological aspects of regeneration despite the major involvement of the  remaining vascular system and the contained hemocytes in this process. To dissect  this process, we analyzed colony blood flow patterns using time-lapse microscopy to  obtain a quantitative description of the velocity, reversal pattern, and average distance  traveled by hemocytes. We also observed that flows present during regeneration are  powered by temporally and spatially synchronized contractions of the terminal  ampullae. In addition, we revised previous studies of B. leachii hematology as well as  asexual development using histological sectioning and compared the role played by  hemocytes during WBR. We found that regeneration starts with a rapid healing  response characterized by hemocyte aggregation and infiltration of immunocytes,  followed by increased activity of hemoblasts, recruitment of macrophage-like cells for  clearing the tissues of debris, and their subsequent disappearance from the circulation  concomitant with the maturation of a single regenerated adult. Overall, we provide a  detailed account of the hematological properties of regenerating B. leachii colonies,  providing novel lines of inquiry toward the decipherment of regeneration in chordates.</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>https://folia.unifr.ch/global/documents/307442</dc:identifier>
  <dc:identifier>https://folia.unifr.ch/documents/307442/files/bla_haa.pdf</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1086/692841</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>License undefined</dc:rights>
  <dc:source>The Biological Bulletin. - 2017, vol. 232, no. 3, p. 143–157</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">Botrylloides leachii</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">hemocytes</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">hemolymph flow</dc:subject>
  <dc:subject xmlns:ns4="xml" ns4:lang="en">histology</dc:subject>
  <dc:subject xmlns:ns5="xml" ns5:lang="en">whole-body regeneration</dc:subject>
  <dc:subject>info:eu-repo/classification/udc/57</dc:subject>
  <dc:title xmlns:ns6="xml" ns6:lang="en">Hematological analysis of the ascidian Botrylloides leachii (Savigny, 1816) during whole-body regeneration</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>