<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Jayol, Aurélie</dc:creator>
  <dc:creator>Poirel, Laurent</dc:creator>
  <dc:creator>André, Catherine</dc:creator>
  <dc:creator>Dubois, Véronique</dc:creator>
  <dc:creator>Nordmann, Patrice</dc:creator>
  <dc:date>2018-10-01</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">The commercial SuperPolymyxin medium (ELITechGroup, Puteaux, France), currently  licensed in Europe, is aimed to screen for either intrinsic or acquired colistin  resistance in Gram negatives. It was tested for screening colistin-resistant Gram- negative rods from bacterial cultures of 145 colistin-resistant and 86 colistin- susceptible Gram-negative rods isolates. It exhibited a sensitivity and specificity of  95.2% (138/145) and 95.3% (82/86), respectively. The sensitivity for the detection of  the 25 isolates carrying plasmid-mediated colistin resistance genes (mcr-1, mcr-2,  mcr-3, and mcr-4) from bacterial cultures was of 100%. It was also evaluated for the  detection of colistin-resistant Gram-negative rods from 57 rectal swab samples (41  clinical rectal swab samples and from 16 spiked rectal swab samples supplemented  with colistin-resistant enterobacterial isolates carrying mcr genes). The overall  sensitivity and specificity were of 100% (33/33) and 90.3% (56/62), respectively. The  detection of isolates carrying plasmid-mediated mcr-like genes from rectal swabs was  achieved with a lowest detection limit of 103 to 104 CFU/mL. The SuperPolymyxin  medium is adapted for screening and detection of colistin-resistant isolates from  bacterial cultures and rectal swab samples, regardless of the level and the  mechanism of colistin resistance. It might be useful in the context of prevention of  outbreaks due to colistin-resistant isolates and for epidemiological surveillance.</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>https://folia.unifr.ch/global/documents/307433</dc:identifier>
  <dc:identifier>https://folia.unifr.ch/documents/307433/files/nor_dcr.pdf</dc:identifier>
  <dc:identifier>https://folia.unifr.ch/documents/307433/files/nor_dcr_sm.pdf</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1016/j.diagmicrobio.2018.05.008</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>License undefined</dc:rights>
  <dc:source>Diagnostic Microbiology and Infectious Disease. - 2018, vol. 92, no. 2, p. 95–101</dc:source>
  <dc:subject>info:eu-repo/classification/udc/57</dc:subject>
  <dc:title xmlns:ns1="xml" ns1:lang="en">Detection of colistin-resistant Gram-negative rods by using the SuperPolymyxin medium</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>