Roccaro-Waldmeyer, Diana M. Girard, Franck Milani, Daniele Vannoni, Elisabetta Prétôt, Laurent Wolfer, David P. Celio, Marco R. 2018 The calcium-binding protein parvalbumin (PV) is a recognized marker of short-axon GABA-ergic neurons in the cortex and the hippocampus. However in addition, PV is expressed by excitatory, glutamatergic neurons in various areas of the brain and spinal cord. Depending on the location of these neurons, loading of their synaptic vesicles with glutamate is mediated by either of three vesicular glutamate transporters (VGlut): VGlut1, VGlut2 or VGlut3. Driven by our interest in one of these glutamatergic/PV-expressing cell clusters – the lateral hypothalamic parvafox nucleus - we investigated the functions of this population of neurons by the selective deletion of VGlut2 expression in PV-expressing cells according to the Cre/Lox-approach. PV- Cre;VGlut2-Lox mutant mice are phenotypically characterized by deficits in locomotion and vocalization, by a decreased thermal nociception, and by an increased social dominance. We conducted a search of the Allen Brain Atlas for regions that might co- express the genes encoding PV and VGlut2, and that might thus contribute to the manifestation of the observed phenotypes. Our survey revealed several structures that could contribute to the deficits in locomotion and vocalization, such as the red, the subthalamic and the deep cerebellar nuclei. It also disclosed that a shift in the balance of afferental glutamatergic neurotransmission to the periaqueductal grey matter might be accountable for the decrease in sensitivity to pain and for the increase in social dominance. As a whole, this study broadens the state of knowledge about PV- expressing excitatory neurons. application/pdf https://folia.unifr.ch/global/documents/307144 https://folia.unifr.ch/documents/307144/files/cel_evd.pdf https://folia.unifr.ch/documents/307144/files/cel_evd_sm.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.3389/fnbeh.2018.00146 info:eu-repo/semantics/openAccess License undefined Frontiers in Behavioral Neuroscience. - 2018, vol. 12, p. 146 info:eu-repo/classification/udc/57 Eliminating the VGlut2-dependent glutamatergic transmission of parvalbumin-expressing neurons leads to deficits in locomotion and vocalization, decreased pain sensitivity, and increased dominance http://purl.org/coar/resource_type/c_6501