Chortarea, Savvina Fytianos, Kleanthis Rodriguez-Lorenzo, Laura Petri-Fink, Alke Rothen-Rutishauser, Barbara 2018-05-01 Aim: The distribution and impact of aerosol-delivered gold nanoparticles (AuNPs) functionalized with a mixture of aminated-polyvinyl alcohol and amino-PEG ([polyvinyl alcohol/PEG]-NH2) upon repeated administration onto a 3D lung model were explored. Materials & methods: AuNPs were aerosolized and uptake and epithelial translocation was assessed by inductively coupled plasma optical-emission spectroscopy, flow cytometry and electron microscopy. In addition, cytotoxicity, apoptosis and proinflammation were evaluated. Results: Repeated AuNP aerosolization resulted in NP accumulation in macrophages and epithelial cells. Dendritic cells demonstrated substantial NP internalization after single administration which was reduced in later time points. No cytotoxicity or proinflammation was observed but after 96 h significant apoptosis was induced by the polymer coating. Conclusion: These results indicate the importance of repeated exposures in addressing potential effects of NPs. application/pdf https://folia.unifr.ch/global/documents/307138 https://folia.unifr.ch/documents/307138/files/fin_dpc.pdf https://folia.unifr.ch/documents/307138/files/fin_dpc_sm.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.2217/nnm-2017-0358 info:eu-repo/semantics/openAccess License undefined Nanomedicine. - 2018, vol. 13, no. 10, p. 1169–1185 info:eu-repo/classification/udc/54 Distribution of polymer-coated gold nanoparticles in a 3D lung model and indication of apoptosis after repeated exposure http://purl.org/coar/resource_type/c_6501