Zhang, Yuqin Nasser, Victoria Pisanty, Odelia Omary, Moutasem Wulff, Nikolai Donato, Martin Di Tal, Iris Hauser, Felix Hao, Pengchao Roth, Ohad Fromm, Hillel Schroeder, Julian I. Geisler, Markus Nour-Eldin, Hussam Hassan Shani, Eilon 2018-10-11 Transport of signaling molecules is of major importance for regulating plant growth, development, and responses to the environment. A prime example is the spatial- distribution of auxin, which is regulated via transporters to govern developmental patterning. A critical limitation in our ability to identify transporters by forward genetic screens is their potential functional redundancy. Here, we overcome part of this functional redundancy via a transportome, multi-targeted forward-genetic screen using artificial-microRNAs (amiRNAs). We generate a library of 3000 plant lines expressing 1777 amiRNAs, designed to target closely homologous genes within subclades of transporter families and identify, genotype and quantitatively phenotype, 80 lines showing reproducible shoot growth phenotypes. Within this population, we discover and characterize a strong redundant role for the unstudied ABCB6 and ABCB20 genes in auxin transport and response. The unique multi-targeted lines generated in this study could serve as a genetic resource that is expected to reveal additional transporters. application/pdf https://folia.unifr.ch/global/documents/307088 https://folia.unifr.ch/documents/307088/files/gei_tsa.pdf https://folia.unifr.ch/documents/307088/files/gei_tsa_sm.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-018-06410-y info:eu-repo/semantics/openAccess License undefined Nature Communications. - 2018, vol. 9, no. 1, p. 4204 info:eu-repo/classification/udc/57 A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport http://purl.org/coar/resource_type/c_6501