Tanasescu, Radu Mettal, Ute Colom, Adai Roux, Aurélien Zumbuehl, Andreas 2018-01-09 Giant vesicles (GVs) are widely-used model systems for biological membranes. The formulation of these vesicles, however, can be problematic and artifacts, such as degraded molecules or left-over oil, may be present in the final liposomes. The rapid formulation of a high number of artifact-free vesicles of uniform size using standard laboratory equipment is, therefore, highly desirable. Here, the gentle hydration method of glass bead-supported thin lipid films has been enhanced by adding a vortexing step. This led to the formulation of a uniform population of giant vesicles. Batches of glass beads coated with different lipids can be combined to produce vesicles of hybrid lipid compositions. This method represents a stable approach to rapidly generate giant vesicles. application/pdf https://folia.unifr.ch/global/documents/306541 https://folia.unifr.ch/documents/306541/files/zum_frf.pdf https://folia.unifr.ch/documents/306541/files/zum_frf_sm.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.3390/polym10010054 info:eu-repo/semantics/openAccess License undefined Polymers. - 2018, vol. 10, no. 1, p. 54 info:eu-repo/classification/udc/54 Facile and rapid formation of giant vesicles from glass beads http://purl.org/coar/resource_type/c_6501