Aurore, Valerie Caldana, Fabienne Blanchard, Marianne Kharoubi-Hess, Solange Lannes, Nils Mantel, Pierre-Yves Filgueira, Luis Walch, Michael 2018-02-01 Bone infections are difficult to treat and can lead to severe tissue destruction. Acute bone infections are usually caused by Staphylococcus aureus. Osteoclasts, which belong to the monocyte/macrophage lineage, are the key cells in bone infections. They are not well equipped for killing bacteria and may serve as a reservoir for bacterial pathogens. Silver has been known for centuries for its bactericidal activity. Here, we investigated the bactericidal effects of nano-silver particles in bacteria infected human osteoclasts. We found that nano-silver in per se non-toxic concentration enhanced the bactericidal activity in osteoclasts against intracellular Methicillin-resistant, virulent Staphylococcus aureus. The reduced bacterial survival in nano-silver pretreated cells correlated with increased reactive oxygen responses towards the invading pathogens. Overall, these results indicate that nano-silver compounds should be considered as an effective treatment and prevention option for bacterial bone and orthopedic implant infections. application/pdf https://folia.unifr.ch/global/documents/306507 https://folia.unifr.ch/documents/306507/files/fil_sni.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.nano.2017.11.006 info:eu-repo/semantics/openAccess License undefined Nanomedicine: Nanotechnology, Biology and Medicine. - 2018, vol. 14, no. 2, p. 601–607 info:eu-repo/classification/udc/57 Silver-nanoparticles increase bactericidal activity and radical oxygen responses against bacterial pathogens in human osteoclasts http://purl.org/coar/resource_type/c_6501