Kröpfl, J. M. Spengler, C. M. Frobert, Aurélien Ajalbert, Guillaume Giraud, Marie-Noëlle 2018-01-01 Given the importance of peripheral blood haematopoietic stem and progenitor cells (HPCs) in post-acute regeneration after acute myocardial infarction (MI), the aim of the present study was to investigate the number and secondary replating capacity/self-renewal ability of HPCs in peripheral blood before and 2 weeks after MI. In female Lewis inbred rats (n = 9), MI was induced by ligation of the left coronary artery, and another nine underwent sham surgery, without ligation, for control purposes. Myocardial infarction was confirmed by troponin I concentrations 24 h after surgery. Peripheral blood was withdrawn and fractional shortening and ejection fraction of the left ventricle were assessed before (day 0) and 14 days after MI or sham surgery (day 14). After mononuclear cell isolation, primary and secondary functional colony-forming unit granulocyte–macrophage (CFU-GM) assays were performed in order to detect the kinetics of functional HPC colony counts and cell self- renewal ability in vitro. The CFU-GM counts and cell self-renewal ability remained unchanged (P < 0.05) in both groups at day 14, without interaction between groups. In the intervention group, higher day 0 CFU-GM counts showed a relationship to lower fractional shortening on day 14 (ρ = −0.82; P < 0.01). Myocardial infarction did not negatively affect circulating HPC self-renewal ability, which suggests a constant regenerative potential in the post-acute phase. A relationship of cardiac contractile function 14 days after MI with circulating CFU-GM counts on day 0 might imply functional colony count as a predictive factor for outcome after infarction. application/pdf https://folia.unifr.ch/global/documents/306340 https://folia.unifr.ch/documents/306340/files/gir_mia.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1113/EP086643 info:eu-repo/semantics/openAccess License undefined Experimental Physiology. - 2018, vol. 103, no. 1, p. 1–8 functional hematopoietic colony count self-renewal ability myocardial infarction info:eu-repo/classification/udc/57 Myocardial infarction does not affect circulating haematopoietic stem and progenitor cell self‐renewal ability in a rat model http://purl.org/coar/resource_type/c_6501