König, Désirée Page, Lionel Chassot, Bérénice Jaźwińska, Anna 2018-01-15 The skeleton of adult zebrafish fins comprises lepidotrichia, which are dermal bones of the rays, and actinotrichia, which are non-mineralized spicules at the distal margin of the appendage. Little is known about the regenerative dynamics of the actinotrichia- specific structural proteins called Actinodins. Here, we used immunofluorescence analysis to determine the contribution of two paralogous Actinodin proteins, And1/2, in regenerating fins. Both proteins were detected in the secretory organelles in the mesenchymal cells of the blastema, but only And1 was detected in the epithelial cells of the wound epithelium. The analysis of whole mount fins throughout the entire regenerative process and longitudinal sections revealed that And1-positive fibers are complementary to the lepidotrichia. The analysis of another longfin fish, a gain-of- function mutation in the potassium channel kcnk5b, revealed that the long-fin phenotype is associated with an extended size of actinotrichia during homeostasis and regeneration. Finally, we investigated the role of several signaling pathways in actinotrichia formation and maintenance. This revealed that the pulse-inhibition of either TGFβ/Activin-βA or FGF are sufficient to impair deposition of Actinodin during regeneration. Thus, the dynamic turnover of Actinodin during fin regeneration is regulated by multiple factors, including the osteoblasts, growth rate in a potassium channel mutant, and instructive signaling networks between the epithelium and the blastema of the regenerating fin. application/pdf https://folia.unifr.ch/global/documents/306333 https://folia.unifr.ch/documents/306333/files/jaz_dar.pdf https://folia.unifr.ch/documents/306333/files/jaz_dar_sm.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ydbio.2017.07.024 info:eu-repo/semantics/openAccess License undefined Developmental Biology. - 2018, vol. 433, no. 2, p. 416–432 Actinodin Blastema Lepidotrichia Wound epithelium: Osteoblasts: Extracellular matrix info:eu-repo/classification/udc/57 Dynamics of actinotrichia regeneration in the adult zebrafish fin http://purl.org/coar/resource_type/c_6501