Thriene, Kerstin Grüning, Björn Andreas Bornert, Olivier Erxleben, Anika Leppert, Juna Athanasiou, Ioannis Weber, Ekkehard Kiritsi, Dimitra Nyström, Alexander Reinheckel, Thomas Backofen, Rolf Has, Cristina Bruckner-Tuderman, Leena Dengjel, Jörn 2018-01-11 The extracellular matrix protein collagen VII is part of the microenvironment of stratified epithelia and critical in organismal homeostasis. Mutations in the encoding gene COL7A1 lead to the skin disorder dystrophic epidermolysis bullosa (DEB), are linked to skin fragility and progressive inflammation-driven fibrosis that facilitates aggressive skin cancer. So far, these changes have been linked to mesenchymal alterations, the epithelial consequences of collagen VII loss remaining under- addressed. As epithelial dysfunction is a principal initiator of fibrosis, we performed a comprehensive transcriptome and proteome profiling of primary human keratinocytes to generate global and detailed images of dysregulated epidermal molecular pathways linked to loss of collagen VII. These revealed downregulation of interaction partners of collagen VII on mRNA and protein level, but also increased abundance of S100 pro- inflammatory proteins in primary DEB keratinocytes. Increased TGF-β signaling due to loss of collagen VII was associated with enhanced activity of lysosomal proteases in both keratinocytes and skin of collagen VII-deficient individuals. Thus, loss of a single structural protein, collagen VII, has extra- and intracellular consequences, resulting in inflammatory processes that enable tissue destabilization and promote keratinocyte- driven, progressive fibrosis. application/pdf https://folia.unifr.ch/global/documents/306229 https://folia.unifr.ch/documents/306229/files/den_coa.pdf https://folia.unifr.ch/documents/306229/files/den_coa_sm.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1074/mcp.RA117.000437 info:eu-repo/semantics/openAccess License undefined Molecular & Cellular Proteomics. - 2018, vol. 17, no. 4, p. 565-579 info:eu-repo/classification/udc/57 Combinatorial omics analysis reveals perturbed lysosomal homeostasis in collagen VII-deficient keratinocytes http://purl.org/coar/resource_type/c_6501