Bohmer, Nils Demarmels, Nino Tsolaki, Elena Gerken, Lukas Keevend, Kerda Bertazzo, Sergio Lattuada, Marco Herrmann, Inge K. 2017-09-06 The magnetic separation of pathogenic compounds from body fluids is an appealing therapeutic concept. Recently, removal of a diverse array of pathogens has been demonstrated using extracorporeal dialysis-type devices. The contact time between the fluid and the magnetic beads in such devices is limited to a few minutes. This poses challenges, particularly if large compounds such as bacteria or cells need to be removed. Here, we report on the feasibility to remove cells from body fluids in a continuous dialysis type of setting. We assessed tumor cell removal efficiencies from physiological fluids with or without white blood cells using a range of different magnetic bead sizes (50–4000 nm), concentrations, and contact times. We show that tumor cells can be quantitatively removed from body fluids within acceptable times (1– 2 min) and bead concentrations (0.2 mg per mL). We further present a mathematical model to describe the minimal bead number concentration needed to remove a certain number of cells, in the presence of competing nonspecific uptake. The present study paves the way for investigational studies to assess the therapeutic potential of cell removal by magnetic blood purification in a dialysis-like setting. application/pdf https://folia.unifr.ch/global/documents/305931 https://folia.unifr.ch/documents/305931/files/lat_rcb.pdf https://folia.unifr.ch/documents/305931/files/lat_rcb_sm.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1021/acsami.7b10140 info:eu-repo/semantics/openAccess License undefined ACS Applied Materials & Interfaces. - 2017, vol. 9, no. 35, p. 29571–29579 info:eu-repo/classification/udc/54 Removal of cells from body fluids by magnetic separation in batch and continuous mode: influence of bead size, concentration, and contact time http://purl.org/coar/resource_type/c_6501