<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Fytianos, Kleanthis</dc:creator>
  <dc:creator>Chortarea, Savvina</dc:creator>
  <dc:creator>Rodriguez-Lorenzo, Laura</dc:creator>
  <dc:creator>Blank, Fabian</dc:creator>
  <dc:creator>Garnier, Christophe von</dc:creator>
  <dc:creator>Petri-Fink, Alke</dc:creator>
  <dc:creator>Rothen-Rutishauser, Barbara</dc:creator>
  <dc:date>2017-01-24</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">Nanocarrier design combined with pulmonary drug delivery holds great promise for the  treatment of respiratory tract disorders. In particular, targeting of dendritic cells that are  key immune cells to enhance or suppress an immune response in the lung is a  promising approach for the treatment of allergic diseases. Fluorescently encoded  poly(vinyl alcohol) (PVA)-coated gold nanoparticles, functionalized with either negative  (−COO–) or positive (−NH3+) surface charges, were functionalized with a DC-SIGN  antibody on the particle surface, enabling binding to a dendritic cell surface receptor. A  3D coculture model consisting of epithelial and immune cells (macrophages and  dendritic cells) mimicking the human lung epithelial tissue barrier was employed to  assess the effects of aerosolized AuNPs. PVA-NH2 AuNPs showed higher uptake  compared to that of their −COOH counterparts, with the highest uptake recorded in  macrophages, as shown by flow cytometry. None of the AuNPs induced cytotoxicity or  necrosis or increased cytokine secretion, whereas only PVA-NH2 AuNPs induced  higher apoptosis levels. DC-SIGN AuNPs showed significantly increased uptake by  monocyte-derived dendritic cells (MDDCs) with subsequent activation compared to  non-antibody-conjugated control AuNPs, independent of surface charge. Our results  show that DC-SIGN conjugation to the AuNPs enhanced MDDC targeting and  activation in a complex 3D lung cell model. These findings highlight the potential of  immunoengineering approaches to the targeting and activation of immune cells in the  lung by nanocarriers.</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>https://folia.unifr.ch/global/documents/305564</dc:identifier>
  <dc:identifier>https://folia.unifr.ch/documents/305564/files/rot_adf.pdf</dc:identifier>
  <dc:identifier>https://folia.unifr.ch/documents/305564/files/rot_adf_sm.pdf</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1021/acsnano.6b06061</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>License undefined</dc:rights>
  <dc:source>ACS Nano. - 2017, vol. 11, no. 1, p. 375–383</dc:source>
  <dc:subject>info:eu-repo/classification/udc/54</dc:subject>
  <dc:title xmlns:ns1="xml" ns1:lang="en">Aerosol delivery of functionalized gold nanoparticles target and activate dendritic cells in a 3D lung cellular model</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>