Dobias, Jan Poirel, Laurent Nordmann, Patrice 2017-03-23 To evaluate whether acquired resistance to cationic antimicrobial peptides (CAMP) group molecules, being normal components of the human immune system, may select co-resistance to antibiotic peptides such as polymyxins, considering they share the same mechanism of action. We aimed to evaluate strains producing the recently identified plasmid-encoded polymyxin resistance determinant MCR-1, which is a phosphoethanolamine transferase that modifies the lipopolysaccharide structure of Gram-negative bacteria.Methods: In-vitro susceptibility studies using human CAMPs, namely cathelicidin LL-37, α-defensin 5 (HD5) and β-defensin 3 (HDB3), towards MCR-1-producing and colistin-resistant Escherichia coli or Klebsiella pneumoniae were performed.Results: Cross-resistance to CAMPs and colistin mediated by MCR-1 or chromosomal mechanisms was neither observed in E. coli nor in K. pneumoniae.Conclusion: Therefore, the future therapeutic development of human CAMPs may likely not be impeded by the spread of MCR-1 plasmid-mediated resistance to polymyxins, at least in E. coli. application/pdf https://folia.unifr.ch/global/documents/305495 https://folia.unifr.ch/documents/305495/files/nor_crh.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cmi.2017.03.015 info:eu-repo/semantics/openAccess License undefined Clinical Microbiology and Infection. - 2017, vol. 23, no. 9, p. 676.e1-676.e5 Colistin Defensins Enterobacteriaceae Escherichia coli Klebsiella pneumoniae MCR-1 info:eu-repo/classification/udc/57 Cross-resistance to human cationic antimicrobial peptides and to polymyxins mediated by the plasmid-encoded MCR-1? http://purl.org/coar/resource_type/c_6501