Zürcher, Evelyne Liu, Jingchun Donato, Martin di Geisler, Markus Müller, Bruno 2016-09-02 Morphogenetic signals control the patterning of multicellular organisms. Cytokinins are mobile signals that are perceived by subsets of plant cells. We found that the responses to cytokinin signaling during Arabidopsis development are constrained by the transporter PURINE PERMEASE 14 (PUP14). In our experiments, the expression of PUP14 was inversely correlated to the cytokinin signaling readout. Loss of PUP14 function allowed ectopic cytokinin signaling accompanied by aberrant morphogenesis in embryos, roots, and the shoot apical meristem. PUP14 protein localized to the plasma membrane and imported bioactive cytokinins, thus depleting apoplastic cytokinin pools and inhibiting perception by plasma membrane–localized cytokinin sensors to create a sink for active ligands. We propose that the spatiotemporal cytokinin sink patterns established by PUP14 determine the cytokinin signaling landscape that shapes the morphogenesis of land plants. application/pdf https://folia.unifr.ch/global/documents/305261 https://folia.unifr.ch/documents/305261/files/gei_pdr.pdf https://folia.unifr.ch/documents/305261/files/gei_pdr_sm.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1126/science.aaf7254 info:eu-repo/semantics/openAccess License undefined Science. - 2016, vol. 353, no. 6303, p. 1027–1030 info:eu-repo/classification/udc/57 Plant development regulated by cytokinin sinks http://purl.org/coar/resource_type/c_6501