Krahn, Thomas Wibberg, Daniel Maus, Irena Winkler, Anika Bontron, Séverine Sczyrba, Alexander Nordmann, Patrice Pühler, Alfred Poirel, Laurent Schlüter, Andreas 2016-01-05 The species Acinetobacter baumannii is one of the most important multidrug-resistant human pathogens. To determine its virulence and antibiotic resistance determinants, the genome of the nosocomial <em>bla</em>_NDM-1-positive A. baumannii strain R2090 originating from Egypt was completely sequenced. Genome analysis revealed that strain R2090 is highly related to the community-acquired Australian A. baumannii strain D1279779. The two strains belong to sequence type 267 (ST267). Isolate R2090 harbored the chromosomally integrated transposon Tn<em>125</em> carrying the carbapenemase gene <em>bla</em>_{NDM- 1} that is not present in the D1279779 genome. To test the transferability of the metallo-β-lactamase (MBL) gene region, the clinical isolate R2090 was mated with the susceptible A. baumannii recipient CIP 70.10, and the carbapenem-resistant derivative R2091 was obtained. Genome sequencing of the R2091 derivative revealed that it had received an approximately 66-kb region comprising the transposon Tn<em>125</em> embedding the <em>bla</em>_NDM-1 gene. This region had integrated into the chromosome of the recipient strain CIP 70.10. From the four known mechanisms for horizontal gene transfer (conjugation, outer membrane vesicle- mediated transfer, transformation, and transduction), conjugation could be ruled out, since strain R2090 lacks any plasmid, and a type IV secretion system is not encoded in its chromosome. However, strain R2090 possesses three putative prophages, two of which were predicted to be complete and therefore functional. Accordingly, it was supposed that the transfer of the resistance gene region from the clinical isolate R2090 to the recipient occurred by general transduction facilitated by one of the prophages present in the R2090 genome. Hence, phage-mediated transduction has to be taken into account for the dissemination of antibiotic resistance genes within the species A. baumannii. application/pdf https://folia.unifr.ch/global/documents/305041 https://folia.unifr.ch/documents/305041/files/nor_itc.pdf https://folia.unifr.ch/documents/305041/files/nor_itc_sm.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1128/AAC.00124-16 info:eu-repo/semantics/openAccess License undefined Antimicrobial Agents and Chemotherapy. - 2016, vol. 60, no. 5, p. 3032–3040 info:eu-repo/classification/udc/57 Intraspecies transfer of the chromosomal Acinetobacter baumannii blaNDM-1 carbapenemase gene http://purl.org/coar/resource_type/c_6501